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Team:IIT Madras/Notebook/Protocols
From 2011.igem.org
(Difference between revisions)
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<div style="position:relative; top:50px;"> | <div style="position:relative; top:50px;"> | ||
<h1>Protocols</h1><br/> | <h1>Protocols</h1><br/> | ||
| + | <div style="position:relative; left:16px;"> | ||
<div id="protocol1">Preperation of competent DH5alpha cells</div> | <div id="protocol1">Preperation of competent DH5alpha cells</div> | ||
<div id="protocol1_content"></div> | <div id="protocol1_content"></div> | ||
<div id="protocol2">Transformation</div> | <div id="protocol2">Transformation</div> | ||
| - | <div id="protocol2_content"></div> | + | <div id="protocol2_content"></div> |
<div id="protocol3">Miniprep using alkaline lysis buffers</div> | <div id="protocol3">Miniprep using alkaline lysis buffers</div> | ||
<div id="protocol3_content"></div> | <div id="protocol3_content"></div> | ||
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<div id="protocol9">Ligation</div> | <div id="protocol9">Ligation</div> | ||
<div id="protocol9_content"></div> | <div id="protocol9_content"></div> | ||
| + | </div> | ||
</html> | </html> | ||
Revision as of 20:15, 5 October 2011
Protocols
Preperation of competent DH5alpha cells
Transformation
Miniprep using alkaline lysis buffers
Agarose Gel Electrohoresis
Restriction digestion
Gel Elusion
PCR
PCR_purification
Ligation
