Team:Peking R/Notebook/CSB

From 2011.igem.org

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   <table width="600" border="0" cellspacing="0" cellpadding="0">
   <table width="600" border="0" cellspacing="0" cellpadding="0">
     <tr>
     <tr>
-
       <th height="38" colspan="5" bgcolor="#DCF8A9" class="notbookmaintitle" scope="col">Chen Shuobing's Notebook</th>
+
       <th height="38" colspan="5" bgcolor="#FFFFFF" class="notbookmaintitle" scope="col">Chen Shuobing's Notebook</th>
     </tr>
     </tr>
     <tr>
     <tr>
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       <th colspan="5" bgcolor="#DCF8A9" class="notbookmaintitle" scope="col"><hr /></th>
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       <th colspan="5" bgcolor="#FFFFFF" class="notbookmaintitle" scope="col"><hr /></th>
     </tr>
     </tr>
     <tr>
     <tr>
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       <th width="16" height="94" bgcolor="#DCF8A9" scope="row"><p>&nbsp;</p>
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       <th width="3" height="70" bgcolor="#FFFFFF" scope="row"><p>&nbsp;</p>
       <p>&nbsp;</p></th>
       <p>&nbsp;</p></th>
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       <th colspan="2" bgcolor="#DCF8A9" scope="row"><span class="Iam"><span class="selfstatement3">I am the leader of iGEM 2011 Peking_R team. I take part in designing project and the modeling module.</span></span><span class="selfstatement3"></span></th>
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       <th colspan="2" bgcolor="#FFFFFF" scope="row"><p>I am the leader of iGEM 2011 Peking_R team. I take part in designing project and the modeling module</p></th>
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       <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Peking_R/Team/CSB"><img src="https://static.igem.org/mediawiki/2011/a/a2/PekingR_robin11.jpg" alt="" width="100" height="140" /></a><a href="https://2011.igem.org/Team:Peking_R/Team/ZZR"></a></p></th>
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       <th width="108" rowspan="6" bgcolor="#FFFFFF" scope="row"><p>&nbsp;</p></th>
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       <th width="20" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
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       <th width="16" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
     </tr>
     </tr>
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     <tr>
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       <th rowspan="40" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
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       <th rowspan="40" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
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       <th width="29" height="29" bgcolor="#DCF8A9" scope="row"><img src="https://static.igem.org/mediawiki/2011/0/0c/PekingR_PDF_download_logo.png" alt="" width="30" height="29" /></th>
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       <th width="46" height="66" bgcolor="#FFFFFF" scope="row"><img src="https://static.igem.org/mediawiki/2011/0/0c/PekingR_PDF_download_logo.png" alt="" width="30" height="29" /></th>
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       <th width="410" nowrap="nowrap" bgcolor="#DCF8A9" class="download" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook/ZZR" class="clickteam">Download his note</a></th>
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       <th width="427" nowrap="nowrap" bgcolor="#FFFFFF" class="download" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook/ZZR" class="clickteam">Download his note</a></th>
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       <th rowspan="40" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
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       <th rowspan="40" bgcolor="#FFFFFF" scope="row">&nbsp;</th>
     </tr>
     </tr>
     <tr>
     <tr>
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       <th height="23" colspan="2" bgcolor="#DCF8A9" class="name" scope="row">Contents:</th>
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       <th height="23" colspan="2" bgcolor="#FFFFFF" class="name" scope="row">Contents:</th>
     </tr>
     </tr>
     <tr>
     <tr>
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       <th height="8" colspan="2" bgcolor="#DCF8A9" scope="row"><hr /></th>
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       <th height="8" colspan="2" bgcolor="#FFFFFF" scope="row"><hr /></th>
     </tr>
     </tr>
     <tr>
     <tr>
-
       <th colspan="2" bgcolor="#DCF8A9" class="July"> <a href="#July" class="clickteam">*July,2011</a></th>
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     </tr>
     </tr>
     <tr>
     <tr>
-
      <th colspan="2" bgcolor="#DCF8A9" class="July" scope="row"><a href="#Augest" class="clickteam">*Augest,2011</a></th>
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       <th colspan="3" align="left" bgcolor="#FFFFFF" scope="row"><p><strong>6.24-6.28</strong><br />
-
    </tr>
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Training the experiment of cloning skill.</p>
-
    <tr>
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        <p><strong>6.29-7.5</strong><br />
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       <th colspan="3" bgcolor="#DCF8A9" class="July" scope="row"><a href="#September" class="clickteam">*September,2011</a></th>
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           Test the instrument, such as the flow  cytometers, ELISA Reader, fluorescence microscope.<br />
-
    </tr>
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         Test the M63 and M9 minimal medium.</p>
-
    <tr>
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         <p><strong>7.6-7.12</strong><br />
-
      <th colspan="3" bgcolor="#DCF8A9" class="July" scope="row"><a href="#October" class="clickteam">*October,2011</a></th>
+
          Characterize the dose response curve of original  TPP hammerhead ribozyme.<br />
-
    </tr>
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        Make sure the sequence of bistable switch. </p>
-
    <tr>
+
        <p><strong>7.13-7.21</strong><br />
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
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          Construct the TPP hammerhead ribozyme with  the promoter pBAD, but fail at last.<br />
-
    </tr>
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          Get the code of the RBS calculator.<br />
-
    <tr>
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        Test the performance of GFP reporter  library, and fit with the RBS calculator.</p>
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
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        <p><strong>7.22-7.25</strong><br />
-
    </tr>
+
        Improve the algorithm of the RBS  calculator.</p>
-
    <tr>
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        <p><strong>7.26-7.31</strong><br />
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">July
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        Attended iGEM 2011 China Meetup and had a few days’ holiday.</p>
-
      <a name="July" id="July"></a>        <hr /></th>
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         <p><strong>8.1-8.6</strong><br />
-
    </tr>
+
          Construct the library of CI fused GFP with  different RBS sequence.</p>
-
    <tr>
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         <p><strong>8.7-8.11</strong><br />
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
+
          Test the performance of library of bistable  switch, and get some cloning with the appropriate ratio of green and red.<br />
-
        <tr>
+
          Further characterize the performance of  bistable switch using the flow cytometer.</p>
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
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         <p><strong>8.12-8.15</strong><br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
+
          Characterize the AND gate performance in LB  medium and M9 medium under different induced OD value.</p>
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
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         <p><strong>8.16-8.23</strong><br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
+
           Characterize the TPP hammerhead ribozyme  with the pBAD promoter.<br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
+
           Characterize the AND gate with the 1G1  theophylline riboswitch.<br />
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
+
           Characterize the engineered c-di-GMP  ribozyme.</p>
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
+
         <p><strong>8.24-8.31</strong><br />
-
        </tr>
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           Characterize the AND gate with different  RNA controller.<br />
-
        <tr>
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           Characterize the theophylline riboswitch  and TPP hammerhead ribozyme.</p>
-
          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
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         <p><strong>9.1-9.8</strong><br />
-
          <td bgcolor="#DCF8A9" class="sunday"><span class="sunday">-</span></td>
+
          Confirm the performance of the exist RNA  controller with the GFP and the CI fused GFP</p>
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
         <p><strong>9.9-9.17</strong><br />
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
          Improving the coding of the RBS calculator  to fit the experiment result and make the final coding.</p>
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
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         <p><strong>9.18-9.28</strong><br />
-
          <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d701" class="clickteam">1</a></td>
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           Characterize the AND gate and fix with the  modeling.</p>
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d702" class="clickteam">2</a></td>
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         <p><strong>9.29-10.4</strong><br />
-
        </tr>
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          Characterize the bistable switch with the  TPP hammerhead ribozyme.<br />
-
        <tr class="clickteam">
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       Wiki writing.</p></th>
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#703" class="clickteam">3</a></span></th>
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-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d704" class="clickteam">4</a></span></td>
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-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d705" class="clickteam">5</a></span></td>
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          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d708" class="clickteam">8</a></span></td>
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          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d707" class="clickteam">9</a></span></td>
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          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d710" class="clickteam">10</a></span></th>
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          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d717" class="clickteam">17</a></span></th>
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          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d723" class="clickteam">23</a></span></td>
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          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d724" class="clickteam">24</a></span></th>
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          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d729" class="clickteam">29</a></span></td>
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          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d730" class="clickteam">30</a></span></td>
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          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d731" class="clickteam">31</a></span></th>
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          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
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          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
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          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
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-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
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-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
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          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
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        </tr>
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      </table></th>
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    </tr>
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      <th height="15" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><hr /></th>
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    <tr>
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      <th height="16" colspan="3" bgcolor="#DCF8A9" class="download" scope="row"><a href="#topjuly" class="clickteam">[Top]</a></th>
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-
    </tr>
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-
    <tr>
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-
      <th height="31" colspan="3" bgcolor="#DCF8A9" class="download" scope="row">&nbsp;</th>
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    </tr>
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    <tr>
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-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">7.1--7.7<a name="d701" id="d701"></a><a name="d702" id="d702"></a><a name="d703" id="d703"></a><a name="d704" id="d704"></a><a name="d705" id="d705"></a><a name="d706" id="d706"></a><a name="d707" id="d707"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
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-
      <p>&nbsp;</p>
+
-
      <p class="selfstatement3"><span class="selfstatement3">7.8-7.14<a name="d708" id="d708"></a><a name="d709" id="d709"></a><a name="d710" id="d710"></a><a name="d711" id="d711"></a><a name="d712" id="d712"></a><a name="d713" id="d713"></a><a name="d714" id="d714"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p class="selfstatement3"><span class="selfstatement3">7.15-7.20<a name="d715" id="d715"></a><a name="d716" id="d716"></a><a name="d717" id="d717"></a><a name="d718" id="d718"></a><a name="d719" id="d719"></a><a name="d720" id="d720"></a></span></p>
+
-
      <p>&nbsp;</p>
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-
      <p>&nbsp;</p>
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-
      <p><span class="selfstatement3">7.21-7.28<a name="d721" id="d721"></a><a name="d722" id="d722"></a><a name="d723" id="d723"></a><a name="d724" id="d724"></a><a name="d725" id="d725"></a><a name="d726" id="d726"></a><a name="d727" id="d727"></a><a name="d728" id="d728"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p class="selfstatement3"><span class="selfstatement3">7.29-7.31<a name="d729" id="d729"></a><a name="d730" id="d730"></a><a name="d731" id="d731"></a></span></p>
+
-
      <p>&nbsp;</p></th>
+
-
    </tr>
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-
    <tr>
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-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">Augest<a name="Augest" id="Augest"></a></th>
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-
    </tr>
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-
    <tr>
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-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
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    </tr>
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    <tr>
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-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
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        <tr>
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          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
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          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
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          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
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          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
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          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
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          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
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          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
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         </tr>
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        <tr>
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          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
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          <td bgcolor="#DCF8A9" class="sunday"><a href="#d801" class="clickteam">1</a></td>
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          <td bgcolor="#DCF8A9" class="sunday"><a href="#d802" class="clickteam">2</a></td>
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          <td bgcolor="#DCF8A9" class="sunday"><a href="#d803" class="clickteam">3</a></td>
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          <td bgcolor="#DCF8A9" class="sunday"><a href="#d804" class="clickteam">4</a></td>
+
-
          <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d805" class="clickteam">5</a></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d806" class="clickteam">6</a></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d807" class="clickteam" >7</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d808" class="clickteam">8</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d809" class="clickteam">9</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d810" class="clickteam">10</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d811" class="clickteam">11</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d812" class="clickteam">12</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d813" class="clickteam">13</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d814"class="clickteam" >14</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d815" class="clickteam">15</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d816" class="clickteam">16</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d817"class="clickteam">17</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d818"class="clickteam">18</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d819"class="clickteam">19</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d820"class="clickteam">20</a></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d821"class="clickteam">21</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d822"class="clickteam">22</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d823"class="clickteam">23</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d824"class="clickteam">24</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d825"class="clickteam">25</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d826"class="clickteam">26</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d827"class="clickteam">27</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d828"class="clickteam">28</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d829"class="clickteam">29</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d830"class="clickteam">30</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d831"class="clickteam">31</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9">-</td>
+
-
        </tr>
+
-
      </table></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#topaugest" class="clickteam">[Top]</a></span></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">8.1-8.5<a name="d801" id="d801"></a><a name="d802" id="d802"></a><a name="d803" id="d803"></a><a name="d804" id="d804"></a></span><a name="d805" id="d805"></a></p>
+
-
         <p>&nbsp;</p>
+
-
<p class="selfstatement3"><span class="selfstatement3">8.6-8.13<a name="d806" id="d806"></a><a name="d807" id="d807"></a><a name="d808" id="d808"></a><a name="d809" id="d809"></a><a name="d810" id="d810"></a><a name="d811" id="d811"></a></span><a name="d812" id="d812"></a><a name="d813" id="d813"></a></p>
+
-
<p>&nbsp;</p>
+
-
<p class="selfstatement3">&nbsp;</p>
+
-
         <p class="selfstatement3"><span class="selfstatement3">8.14-8.18<a name="d814" id="d814"></a><a name="d815" id="d815"></a><a name="d816" id="d816"></a><a name="d817" id="d817"></a><a name="d818" id="d818"></a></span></p>
+
-
        <p class="selfstatement3"><span class="selfstatement3">8.19-8.25<a name="d819" id="d819"></a><a name="d820" id="d820"></a><a name="d821" id="d821"></a><a name="d822" id="d822"></a><a name="d823" id="d823"></a><a name="d824" id="d824"></a><a name="d825" id="d825"></a></span></p>
+
-
         <p class="selfstatement3"><span class="selfstatement3">8.26-8.31<a name="d826" id="d826"></a><a name="d827" id="d827"></a><a name="d828" id="d828"></a><a name="d829" id="d829"></a><a name="d830" id="d830"></a><a name="d831" id="d831"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">September<a name="September" id="September"></a></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row"><hr />
+
-
</th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th height="71" colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
+
-
        </tr>
+
-
        <tr>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d901"class="clickteam">1</a></td>
+
-
          <td valign="middle" bgcolor="#DCF8A9" class="sunday"><a href="#d902"class="clickteam">2</a></td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d903"class="clickteam">3</a></td>
+
-
         </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d904"class="clickteam">4</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d905"class="clickteam">5</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d906"class="clickteam">6</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d907"class="clickteam">7</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d908"class="clickteam">8</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d909"class="clickteam">9</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d910"class="clickteam">10</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d911"class="clickteam">11</a></span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d912"class="clickteam">12</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d913"class="clickteam">13</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d914"class="clickteam">14</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d915"class="clickteam">15</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d916"class="clickteam">16</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d917"class="clickteam">17</a></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d918"class="clickteam">18</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d919"class="clickteam">19</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d920"class="clickteam">20</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d921"class="clickteam">21</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d922"class="clickteam">22</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d923"class="clickteam">23</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d924"class="clickteam">24</a></span></td>
+
-
        </tr>
+
-
        <tr>
+
-
           <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday"><a href="#d925"class="clickteam">25</a></span></th>
+
-
           <td align="center" bgcolor="#DCF8A9"><span class="sunday"><a href="#d926"class="clickteam">26</a></span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d927"class="clickteam">27</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d928"class="clickteam">28</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d929"class="clickteam">29</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d930"class="clickteam">30</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9">-</td>
+
-
         </tr>
+
-
      </table></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><span class="download"><a href="#topseptember" class="clickteam">[Top]</a></span></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row">&nbsp;</th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p class="selfstatement3"><span class="selfstatement3">9.1-9.8<a name="d901" id="d901"></a><a name="d902" id="d902"></a><a name="d903" id="d903"></a><a name="d904" id="d904"></a><a name="d905" id="d905"></a><a name="d906" id="d906"></a><a name="d907" id="d907"></a><a name="d908" id="d908"></a></span></p>
+
-
         <p class="selfstatement3"><span class="selfstatement3">9.9-9.15<a name="d909" id="d909"></a><a name="d910" id="d910"></a><a name="d911" id="d911"></a><a name="d912" id="d912"></a><a name="d913" id="d913"></a><a name="d914" id="d914"></a><a name="d915" id="d915"></a></span></p>
+
-
        <p class="selfstatement3"><span class="selfstatement3">9.16-9.22<a name="d916" id="d916"></a><a name="d917" id="d917"></a><a name="d918" id="d918"></a><a name="d919" id="d919"></a><a name="d920" id="d920"></a><a name="d921" id="d921"></a><a name="d922" id="d922"></a></span></p>
+
-
         <p class="selfstatement3"><span class="selfstatement3">9.23-9.30<a name="d923" id="d923"></a><a name="d924" id="d924"></a><a name="d925" id="d925"></a><a name="d926" id="d926"></a><a name="d927" id="d927"></a><a name="d928" id="d928"></a><a name="d929" id="d929"></a><a name="d930" id="d930"></a></span></p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p>
+
-
      <p>&nbsp;</p></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" class="Julytitle" scope="row">October<a name="October" id="October"></a></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><hr /></th>
+
-
    </tr>
+
-
    <tr>
+
-
      <th colspan="3" bgcolor="#DCF8A9" scope="row"><table width="500" border="0" cellspacing="0" cellpadding="0">
+
-
        <tr>
+
-
           <th bgcolor="#DCF8A9" class="sunday" scope="col">Sun.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Mon.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Tue.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Wed.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Thu.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Fri.</th>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="col">Sat.</th>
+
-
         </tr>
+
-
        <tr>
+
-
          <th bgcolor="#DCF8A9" class="sunday" scope="row">-</th>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td valign="middle" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td bgcolor="#DCF8A9" class="sunday"><a href="#d1001" class="clickteam"><span class="clickteam">1</span></a></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><a href="#d1002" class="clickteam">2</a></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d1003" class="clickteam">3</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><a href="#d1004" class="clickteam">4</a></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">5</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">6</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">7</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">8</span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" ><span class="sunday">9</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">10</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">11</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">12</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">13</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">14</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">15</span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" ><span class="sunday">16</span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">17</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">18</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">19</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">20</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">21</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">22</span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" ><span class="sunday">23</span></th>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">24</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">25</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">26</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">27</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">28</span></td>
+
-
          <td align="center" bgcolor="#DCF8A9"><span class="sunday">29</span></td>
+
-
        </tr>
+
-
        <tr>
+
-
          <th align="center" bgcolor="#DCF8A9" scope="row"><span class="sunday">30</span></th>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">31</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
          <td align="center" bgcolor="#DCF8A9" class="sunday">-</td>
+
-
        </tr>
+
-
      </table></th>
+
-
    </tr>
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      <th colspan="3" align="left" bgcolor="#DCF8A9" scope="row"><p><span class="selfstatement3"><span class="selfstatement3">10.1-10.4</span><a name="d1001" id="d1001"></a><a name="d1002" id="d1002"></a><a name="d1003" id="d1003"></a><a name="d1004" id="d1004"></a></span></p>
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       <th colspan="3" bgcolor="#DCF8A9" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Team/CSB" class="clickteam">==click here to his page==</a></th>
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       <th colspan="3" bgcolor="#FFFFFF" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Team/LYP" class="clickteam">==click here to his page==</a></th>
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       <th colspan="3" bgcolor="#DCF8A9" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook">==click here to return==</a></th>
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       <th colspan="3" bgcolor="#FFFFFF" class="clickteam" scope="row"><a href="https://2011.igem.org/Team:Peking_R/Notebook">==click here to return==</a></th>
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       <th width="146" class="clickteam" scope="col"><span class="clickteam"><a href="https://2011.igem.org/Team:Peking_R/Notebook" class="clickteam">Personal Note</a></span></th>
       <th width="146" class="clickteam" scope="col"><span class="clickteam"><a href="https://2011.igem.org/Team:Peking_R/Notebook" class="clickteam">Personal Note</a></span></th>
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       <th width="252" align="left" class="clickteam" scope="col"><a href="https://2011.igem.org/Team:Peking_R/Notebook/CSB" class="clickteam">Chen Shuobing</a><a name="topjuly" id="topjuly"></a><a name="topaugest" id="topaugest"></a><a name="topseptember" id="topseptember"></a><a name="topoctober" id="topoctober"></a></th>
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       <th width="252" align="left" class="clickteam" scope="col"><a href="https://2011.igem.org/Team:Peking_R/Notebook/LYP" class="clickteam">Chen Shuobing</a><a name="topjuly" id="topjuly"></a><a name="topaugest" id="topaugest"></a><a name="topseptember" id="topseptember"></a><a name="topoctober" id="topoctober"></a></th>
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Revision as of 18:51, 5 October 2011

Template:Https://2011.igem.org/Team:Peking R/bannerhidden Template:Https://2011.igem.org/Team:Peking R/back2 Template:Https://2011.igem.org/Team:Peking R/Notebookbackground 无标题文档

Chen Shuobing's Notebook

 

 

I am the leader of iGEM 2011 Peking_R team. I take part in designing project and the modeling module

 

 
  Download his note  
Contents:

6.24-6.28
Training the experiment of cloning skill.

6.29-7.5
Test the instrument, such as the flow cytometers, ELISA Reader, fluorescence microscope.
Test the M63 and M9 minimal medium.

7.6-7.12
Characterize the dose response curve of original TPP hammerhead ribozyme.
Make sure the sequence of bistable switch.

7.13-7.21
Construct the TPP hammerhead ribozyme with the promoter pBAD, but fail at last.
Get the code of the RBS calculator.
Test the performance of GFP reporter library, and fit with the RBS calculator.

7.22-7.25
Improve the algorithm of the RBS calculator.

7.26-7.31
Attended iGEM 2011 China Meetup and had a few days’ holiday.

8.1-8.6
Construct the library of CI fused GFP with different RBS sequence.

8.7-8.11
Test the performance of library of bistable switch, and get some cloning with the appropriate ratio of green and red.
Further characterize the performance of bistable switch using the flow cytometer.

8.12-8.15
Characterize the AND gate performance in LB medium and M9 medium under different induced OD value.

8.16-8.23
Characterize the TPP hammerhead ribozyme with the pBAD promoter.
Characterize the AND gate with the 1G1 theophylline riboswitch.
Characterize the engineered c-di-GMP ribozyme.

8.24-8.31
Characterize the AND gate with different RNA controller.
Characterize the theophylline riboswitch and TPP hammerhead ribozyme.

9.1-9.8
Confirm the performance of the exist RNA controller with the GFP and the CI fused GFP

9.9-9.17
Improving the coding of the RBS calculator to fit the experiment result and make the final coding.

9.18-9.28
Characterize the AND gate and fix with the modeling.

9.29-10.4
Characterize the bistable switch with the TPP hammerhead ribozyme.
Wiki writing.

 
==click here to his page==
==click here to return==