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| - | {{Team:USTC-China/temp}}
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| - | {{Team:USTC-China/temp/bar4}}
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| - | <html lang="en">
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| - | <style type="text/css">
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| | | | |
| - | a {
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| - | color: #991133;
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| - | text-decoration: none;
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| - | }
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| - | a:hover {
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| - | text-decoration: underline;
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| - | }
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| - |
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| - | .clear {
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| - | clear:both;
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| - | }
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| - |
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| - | #col_left{
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| - | float: left;
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| - | width: 576px;
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| - | padding: 30px 22px;
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| - | margin:0;
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| - | }
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| - | #col_right{
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| - | float: right;
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| - | width: 242px;
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| - | padding: 30px 28px 30px 15px;
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| - | margin:0;
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| - | }
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| - | #col_nav{
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| - | float: left;
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| - | width: 242px;
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| - | padding: 30px 28px 30px 15px;
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| - | margin:0;
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| - | }
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| - |
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| - |
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| - | /* end twitter */
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| - |
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| - |
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| - | .col_list ul{
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| - | list-style-type:none;
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| - | list-style-image:none;
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| - | background-image:none;
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| - |
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| - | }
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| - | .col_list li{
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| - | text-align:center;
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| - | font-family:"Comic Sans MS", cursive;
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| - | font-size: 1.6em;
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| - | padding: 5px 15px;
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| - |
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| - | background-color:transparent;
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| - | }
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| - |
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| - |
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| - |
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| - | /* TEAM PAGE */
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| - | .bio {
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| - | clear:both;
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| - | padding-bottom: 20px;
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| - | border-bottom: 1px solid #ccc;
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| - | }
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| - | .bio img {
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| - | float:left;
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| - | margin-right: 10px;
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| - | }
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| - |
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| - | #videoiframe {
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| - | overflow: hidden;
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| - | margin-top: 20px;
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| - | }
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| - |
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| - | </style>
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| - | </head>
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| - | </html>
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| - |
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| - | <html>
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| - | <head>
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| - | <!-- TODO: add pre/n buttons -->
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| - | <script>
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| - | $(document).ready(function(){
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| - | $('.bio').css('display','none');
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| - | $('.col_list li').css('cursor','pointer');
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| - | $('.col_list li').hover(function() {
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| - | $(this).css('background-color','#91A3ED');
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| - | }, function() {
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| - | $(this).css('opacity','0.6');
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| - | });
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| - |
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| - | $('.col_list li').click(function () {
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| - | $('.bio').hide();
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| - | var name = $(this).attr('id');
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| - | $('#'+name+'bio').fadeIn(500);
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| - |
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| - | });
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| - | });
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| - | </script>
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| - | </head>
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| - |
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| - | <body>
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| - |
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| - | <div id="col_nav">
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| - | <div class="col_list">
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| - | <h2>Weeks</h2>
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| - | <ul>
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| - | <li id="yhl">Jun 28-July 2</li>
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| - | <li id="swl">July 3-July 9</li>
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| - | <li id="zlc">July 10-July 16</li>
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| - | <li id="fzz">July 17-July 23</li>
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| - | <li id="shl">July 24-July 30</li>
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| - | <li id="ml">July 31-Aug 6</li>
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| - | <li id="mps">Aug 7-Aug 13</li>
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| - | <li id="yfl">Aug 14-Aug 20</li>
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| - | <li id="dz">Aug 21-Aug 27</li>
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| - | <li id="lna">Aug 28-Sep 3</li>
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| - | <li id="jhp">Sep 4-Sep 10</li>
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| - | <li id="xlz">Sep 11-Sep 17</li>
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| - | <li id="wyy">Sep 18-Sep 24</il>
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| - | <li id="ytl">Sep 25-Oct 1</li>
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| - | <li id="nw">Oct 2-Oct 8</li>
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| - | <li id="yz">Oct 9-Oct 15</il>
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| - |
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| - | </ul>
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| - | </div><!-- end undergrads -->
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| - | </div>
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| - |
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| - |
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| - |
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| - | <div id="col_left">
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| - |
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| - | <div class="bio" id="yhlbio">
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| - | <h1>Jun 28-July 2</h1>
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| - | </html>
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| - | 2011.6.28
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| - | ----
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| - | cultivate the bacteria of [https://2011.igem.org//Team:USTC-China/cheZ cheZ] deficiency(RP1616),and the Control group(RP437).
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| - | check the resistibility of RP1616 and RP437
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| - | result:both of the two groups are of none resistibility.
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| - | members:Siwei Luo, Fangzhou Zhao, Zhilin Chen...
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| - |
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| - | 2011.6.29
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| - | ----
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| - | check the motility of RP1616 strain and RP437 strain
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| - | cultivate Top10 strain
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| - | result: the bacterial plaque of RP1616 is smaller than that of RP437, implying that RP1616 is of [https://2011.igem.org//Team:USTC-China/cheZ cheZ] deficiency, causing the decreased motility of the bacteria.
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| - |
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| - |
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| - | 6.30
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| - | ----
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| - | prepare for the competent cell of Top10 strain cultivated on 6.29
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| - | extract the genome of Top10 strain and use it as complates to run PCR of CheZ
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| - | result: the concentration of PCR result is too low to continue the experiments.
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| - |
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| - | 7.1
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| - | ----
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| - | conduct PCR of CheZ again
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| - | ligate the CheZ DNA segment to plasmid PSb1C3, and transform it into Top10 competent cells
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| - | result:as the picture shows.
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| - |
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| - | 7.2
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| - | ----
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| - | pick up single colony from the Petri dish to reproduce, in which the competent cells are cultivated and use them colony PCR
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| - | result:The bacteria grow up well on dishes with chloromycetin resistency，verifying that the transformation is successfull
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| - | while the concentration of PCR product is pretty high,demonstrating that the plasmids indeed contains the sagment CheZ.
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| - |
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| - |
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| - | <html>
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="swlbio">
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| - | <h1>July 3-July 9</h1>
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| - | </html>
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| - | 7.3
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| - | ----
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| - | extract the plasmids from the reproduced bacteria and send it to check the base sequence
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| - | result: the concentration of the plasmids extracted is about 500ng/uL
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| - |
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| - | 7.4
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| - | ----
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| - | extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2010 and put them into Top10 strain by transformation
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| - | (LuxPr: Plate 2,24C Terminator : Plate 1,6O)
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| - | result: the bacteria on the plate with Amp resistency grows well
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| - | 7.5
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| - | ----
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| - | pick up single colony from the Petri dish to reproduce in the liquid culture medium
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| - |
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| - | 7.6
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| - | ----
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| - | extract plasmids containing LuxPR and Terminator and make enzyme digestion with EcoR1 and Spe1 in order to validate the existence of the two parts
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| - | recieve part Toggle Switch and rbs-Ci-ter from PKU and conduct transformation
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| - |
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| - | result: Due to the short length of LuxPR and Terminator, the result of the enzyme digestion is hard to examine by electrophoresis.
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| - |
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| - | 7.7
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| - | ----
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| - | result: there are a few colonies with part Toggle switch grown while no colony of rbs-Ci-ter appeared.
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| - |
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| - | 7.8
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| - | ----
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| - | pick up single colony with part Toggle switch .
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| - | extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2011 and put them into Top10 strain by transformation
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| - |
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| - | 7.9
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| - | ----
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| - | pick up single colony with LuxPR & terminator.
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| - | extract plasmids containing toggle switch to conduct PCR.
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| - |
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| - | process PCR with plasmids aptamer-CheZ
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| - | result:as the picture shows.
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| - | the base sequence of CheZ extracted before is proved right.
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| - |
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| - |
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| - |
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| - | <html>
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="zlcbio">
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| - | <h1>July 10-July 16</h1>
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| - | </html>
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| - | 7.10
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| - | ----
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| - | ligate aptamer-CheZ with PSB1C3 plasmids
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| - |
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| - | extract plasmids containing LuxPR and terminator
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| - | result:the concentractions of plasmids LuxPR and terminator are both 600ng/ul
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| - |
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| - | 7.11
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| - | ----
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| - | transform PSB1C3 containing aptamer-CheZ into bacteria and cultivate.
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| - | 7.12
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| - | ----
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| - | carry out Double digestion of toggle switch and pSB1c3 and ligate them together.
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| - |
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| - | pick up single colony from bacteria cultivated yesterday.
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| - |
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| - | result: Both the location and the brightness of the electrophoretic bands correspond with our expectation.
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| - |
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| - | 7.14
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| - | ----
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| - | transform the part rbs-ci-ter sent from PKU again into bacteria.
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| - | check the colors of the colonies containing toggle switch.
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| - | result: the ratio of red vs green is 8:25, verifying the function of toggle switch.
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| - | 7.15
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| - | ----
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| - | pick up the single colony from the plate yesterday and ten hours later extract the plasmids containing Ci-terminator.
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| - | 7.16
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| - | ----
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| - | conduct PCR with the plasmids yesterday to reproduce rbs-ci-Term.
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| - | result:failure
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| - |
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| - |
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| - | <html>
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| - | </div>
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| - |
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| - | <div class="bio" id="fzzbio">
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| - | <h1>July 17-July 23</h1>
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| - | 7.18-7.21
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| - | ----
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| - | ligate the standard part Terminator to the end of toggle switch
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| - | result: we conduct a enzyme digestion with EcoR1 and Pst1 to the final ligation product and the electrophoresis result shows that the ligation is successful.
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="shlbio">
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| - | <h1>July 24-July 30</h1>
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| - | 7.22-7.29
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| - | ----
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| - | perform the following experiments: PCR of rbs-ci-ter, enzyme digestion(E,S),ligation with LuxPR(standard part from IGEM registry digested by E,X),tranformation,plasmids extraction.
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| - | result: the concentration of plasmids with ligation gene is as high as ...
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| - |
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="mlbio">
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| - | <h1>July 31-Aug 6</h1>
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| - | 7.30-8.3
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| - | ----
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| - | PCR of LuxPR-rbs-ci-ter, enzyme digestion(E,S),ligation with LuxPR(standard part from IGEM registry digested by E,X),tranformation,plasmids extraction.
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| - | </div>
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| - |
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| - |
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| - | <div class="bio" id="mpsbio">
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| - | <h1>Aug 7-Aug 13</h1>
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - |
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| - | <div class="bio" id="yflbio">
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| - | <h1>Aug 14-Aug 20</h1>
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| - |
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| - | </div>
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| - |
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| - |
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| - | <div class="bio" id="nwbio">
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| - | <h1></h1>
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| - |
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="ytlbio">
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| - | <h1></h1>
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| - |
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="dzbio">
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| - | <h1></h1>
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| - |
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| - | </div>
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| - |
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| - | <div class="bio" id="lnabio">
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| - | <h1></h1>
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="jhpbio">
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| - | <h1></h1>
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| - |
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| - |
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="yywbio">
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| - | <h1></h1>
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="xlzbio">
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| - | <h1></h1>
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="yzbio">
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| - | <h1></h1>
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - |
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| - | <div class="bio" id="wyybio">
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| - | <h1></h1>
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| - |
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| - | <div class="clear"></div>
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| - | </div>
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| - | </div>
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| - |
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| - | </body>
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| - | </html>
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