Team:HKU-Hong Kong/Parts

From 2011.igem.org

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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;"|Sample Data Page
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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By expressing the tetR-HNS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the 2 tetR binding region (tetO2).Thus in our designed operator- repressor binding, the presence of 2 tetR binding sites are used to enhance the binding of tetR part of the fusion proteins. As the fusion proteins bound to tetO, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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'''Experience:'''
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We have entered the confirmation data for the characterization of the selected constitutive promoters we have used. Of the 4 promoters we have used in the project, our team considered that J23109 or J23116 are more suitable promoters in creating the super silencer repression system.
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These parts are:
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:HKU-Hong_Kong_logo.png|200px|right|frame]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="left"|[http://partsregistry.org/Part:BBa_J23103:Experience '''BBa_J23103''' ]
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''Tell us more about your project. Give us background.  Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="centre"|[http://partsregistry.org/Part:BBa_J23106:Experience '''BBa_J23106''' ]
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|[[Image:HKU-Hong_Kong_team.png|right|frame|Your team picture]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="right"|[http://partsregistry.org/Part:BBa_J23109:Experience'''BBa_J23109''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="right"|[http://partsregistry.org/Part:BBa_J23116:Experience'''BBa_J23116''' ]
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|align="center"|[[Team:HKU-Hong_Kong | Team Example]]
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:HKU-Hong_Kong|Home]]
 
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!align="center"|[[Team:HKU-Hong_Kong/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=HKU-Hong_Kong Official Team Profile]
 
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!align="center"|[[Team:HKU-Hong_Kong/Project|Project]]
 
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!align="center"|[[Team:HKU-Hong_Kong/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:HKU-Hong_Kong/Modeling|Modeling]]
 
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!align="center"|[[Team:HKU-Hong_Kong/Notebook|Notebook]]
 
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!align="center"|[[Team:HKU-Hong_Kong/Safety|Safety]]
 
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!align="center"|[[Team:HKU-Hong_Kong/Attributions|Attributions]]
 
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===Parts===
 
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
 
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<groupparts>iGEM010 HKU-Hong_Kong</groupparts>
 

Latest revision as of 17:30, 5 October 2011

Sample Data Page
Sample Data Parts.png

By expressing the tetR-HNS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the 2 tetR binding region (tetO2).Thus in our designed operator- repressor binding, the presence of 2 tetR binding sites are used to enhance the binding of tetR part of the fusion proteins. As the fusion proteins bound to tetO, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.

Sample Data Table.png

Experience:

We have entered the confirmation data for the characterization of the selected constitutive promoters we have used. Of the 4 promoters we have used in the project, our team considered that J23109 or J23116 are more suitable promoters in creating the super silencer repression system.

These parts are:

[http://partsregistry.org/Part:BBa_J23103:Experience BBa_J23103 ] [http://partsregistry.org/Part:BBa_J23106:Experience BBa_J23106 ] [http://partsregistry.org/Part:BBa_J23109:ExperienceBBa_J23109 ] [http://partsregistry.org/Part:BBa_J23116:ExperienceBBa_J23116 ]
Retrieved from "http://2011.igem.org/Team:HKU-Hong_Kong/Parts"