Team:HKU-Hong Kong/Parts

From 2011.igem.org

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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;"|Sample Data Page
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By expressing the tetR:H-NS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the tetR binding region (tetO2). There are two tet-R binding sites as tet-R form a dimer as in the tet repressor- operator system. As the fusion proteins bound to tet-O, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.
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By expressing the tetR-HNS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the 2 tetR binding region (tetO2).Thus in our designed operator- repressor binding, the presence of 2 tetR binding sites are used to enhance the binding of tetR part of the fusion proteins. As the fusion proteins bound to tetO, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.
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'''Experience:'''
'''Experience:'''
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="left"|[http://partsregistry.org/Part:BBa_J23103 '''BBa_J23103''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="left"|[http://partsregistry.org/Part:BBa_J23103:Experience '''BBa_J23103''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="centre"|[http://partsregistry.org/Part:BBa_J23106 '''BBa_J23106''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="centre"|[http://partsregistry.org/Part:BBa_J23106:Experience '''BBa_J23106''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="right"|[http://partsregistry.org/Part:BBa_J23109'''BBa_J23109''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="right"|[http://partsregistry.org/Part:BBa_J23109:Experience'''BBa_J23109''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="right"|[http://partsregistry.org/Part:BBa_J23116'''BBa_J23116''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="right"|[http://partsregistry.org/Part:BBa_J23116:Experience'''BBa_J23116''' ]
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Latest revision as of 17:30, 5 October 2011

Sample Data Page
Sample Data Parts.png

By expressing the tetR-HNS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the 2 tetR binding region (tetO2).Thus in our designed operator- repressor binding, the presence of 2 tetR binding sites are used to enhance the binding of tetR part of the fusion proteins. As the fusion proteins bound to tetO, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.

Sample Data Table.png

Experience:

We have entered the confirmation data for the characterization of the selected constitutive promoters we have used. Of the 4 promoters we have used in the project, our team considered that J23109 or J23116 are more suitable promoters in creating the super silencer repression system.

These parts are:

[http://partsregistry.org/Part:BBa_J23103:Experience BBa_J23103 ] [http://partsregistry.org/Part:BBa_J23106:Experience BBa_J23106 ] [http://partsregistry.org/Part:BBa_J23109:ExperienceBBa_J23109 ] [http://partsregistry.org/Part:BBa_J23116:ExperienceBBa_J23116 ]
Retrieved from "http://2011.igem.org/Team:HKU-Hong_Kong/Parts"