Team:HKU-Hong Kong/Parts

From 2011.igem.org

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{| style="width:900px;background:#000000;text-align:justify;font-family: georgia, helvetica, arial, sans-serif;color:#ffffff;margin-top:25px;" cellspacing="20"
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[[http://partsregistry.org/Part:BBa_K544001|BBa_K544001 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[http://partsregistry.org/Part:BBa_K544001 '''BBa_K544001''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[[[http://partsregistry.org/BBa_K544002|BBa_K544002 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[http://partsregistry.org/BBa_K544002 '''BBa_K544002''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="right"|[[[http://partsregistry.org/BBa_K544003|BBa_K544003 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="right"|[http://partsregistry.org/BBa_K544003'''BBa_K544003''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[[[http://partsregistry.org/BBa_K544012|BBa_K544012 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[http://partsregistry.org/BBa_K544012'''BBa_K544012''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[[[http://partsregistry.org|BBa_K544011 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[http://partsregistry.org/BBa_K544011 '''BBa_K544011''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="right"|[[[http://partsregistry.org/|BBa_K544013 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="right"|[http://partsregistry.org/BBa_K544013 '''BBa_K544013''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[[[http://partsregistry.org/BBa_K544014|BBa_K544014 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="left"|[http://partsregistry.org/BBa_K544014 '''BBa_K544014''' ]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[[[http://partsregistry.org/BBa_K544015|BBa_K544021 ]]
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;" align="centre"|[http://partsregistry.org/BBa_K544015 '''BBa_K544021''' ]
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Revision as of 14:56, 5 October 2011

Sample Data Page
Sample Data Parts.png

By expressing the tetR:H-NS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the tetR binding region (tetO2). There are two tet-R binding sites as tet-R form a dimer as in the tet repressor- operator system. As the fusion proteins bound to tet-O, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.


Parts that we have created:

(The favorite parts are selected based on the potential to be used in our Super silencer repression system)

[http://partsregistry.org/Part:BBa_K544001 BBa_K544001 ] [http://partsregistry.org/BBa_K544002 BBa_K544002 ] [http://partsregistry.org/BBa_K544003BBa_K544003 ]
[http://partsregistry.org/BBa_K544012BBa_K544012 ] [http://partsregistry.org/BBa_K544011 BBa_K544011 ] [http://partsregistry.org/BBa_K544013 BBa_K544013 ]
[http://partsregistry.org/BBa_K544014 BBa_K544014 ] [http://partsregistry.org/BBa_K544015 BBa_K544021 ]