"
Team:NCTU Formosa/protocol G
From 2011.igem.org
(Difference between revisions)
| (4 intermediate revisions not shown) | |||
| Line 28: | Line 28: | ||
#globalWrapper{ | #globalWrapper{ | ||
| - | background-color: #565659; | + | //background-color: #565659; |
| + | background-image:url(https://static.igem.org/mediawiki/2011/a/ae/Wallpaper2.jpg); | ||
} | } | ||
| Line 179: | Line 180: | ||
} | } | ||
| + | <style type = "text/javascript" language = "JavaScript"> | ||
| + | function out(id_num){ | ||
| + | var oa = document.getElementById(id_num); | ||
| + | if(oa.style.display == "none"){ | ||
| + | oa.style.display = "block"; | ||
| + | } | ||
| + | return false; | ||
| + | } | ||
| + | </script> | ||
/*content*/ | /*content*/ | ||
| Line 298: | Line 308: | ||
<ul id="cm-nav"> | <ul id="cm-nav"> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa">Home</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa">Home</a></li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">Team </a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/members">Members</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/members">Members</a></li> | ||
| Line 305: | Line 315: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">Project</a> |
<ul class="arrow-pad"> | <ul class="arrow-pad"> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/introduction">Introduction</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/introduction">Introduction</a></li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">RNA Thermometer</a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_design">Design</a></li> | ||
| Line 315: | Line 325: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">CI promoter </a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_design">Design</a></li> | ||
| Line 322: | Line 332: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">Carotenoid synthesis pathway</a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_design">Design</a></li> | ||
| Line 328: | Line 338: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">Butanol pathway</a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_design">Design</a></li> | ||
| Line 334: | Line 344: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">Violacein pathway</a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Design</a></li> | ||
| Line 347: | Line 357: | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human Practice</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human Practice</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Attribution</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Attribution</a></li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">Notebook </a> |
<ul> | <ul> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">Protocols</a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li> | ||
| Line 366: | Line 376: | ||
<b>Method:</b> | <b>Method:</b> | ||
| - | + | <p>Culture samples were centrifuged (12500×g, 25°C, 5 min), and the supernatants were analyzed for isobutanol. Isobutanol products were analyzed by GC equipped with flame ionization detector(FID) and a 50-m Ultra-2 column (Hewlett Packard, USA). The separation of alcohol compounds was carried out with a EQUity capirally column (SUPELCO, 60 m; 0.32 mm inside diameter; 1.0 μm film thickness). Filtrated samples were injected in split injection mode (1:10 split ratio).Nitrogen (constant flow 1 mL/min) was used as a carrier gas. The temperature of the injector was 150 °C. The oven program was as follows: 40°C ramp to 100 °C at 20 °C/min, 100 °C for 1 min, ramp to 130 °C at 15 °C/min, 130°C for 4 min.</p> | |
Latest revision as of 13:40, 5 October 2011
Protocols
Gas chromatography (GC) Protocol
Method:Culture samples were centrifuged (12500×g, 25°C, 5 min), and the supernatants were analyzed for isobutanol. Isobutanol products were analyzed by GC equipped with flame ionization detector(FID) and a 50-m Ultra-2 column (Hewlett Packard, USA). The separation of alcohol compounds was carried out with a EQUity capirally column (SUPELCO, 60 m; 0.32 mm inside diameter; 1.0 μm film thickness). Filtrated samples were injected in split injection mode (1:10 split ratio).Nitrogen (constant flow 1 mL/min) was used as a carrier gas. The temperature of the injector was 150 °C. The oven program was as follows: 40°C ramp to 100 °C at 20 °C/min, 100 °C for 1 min, ramp to 130 °C at 15 °C/min, 130°C for 4 min.
