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Team:NCTU Formosa/protocol G
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<ul id="cm-nav"> | <ul id="cm-nav"> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa">Home</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa">Home</a></li> | ||
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/members">Members</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/members">Members</a></li> | ||
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/introduction">Introduction</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/introduction">Introduction</a></li> | ||
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<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/RNA_design">Design</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">CI promoter </a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CI_design">Design</a></li> | ||
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</ul> | </ul> | ||
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<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/CSP_design">Design</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/BP_design">Design</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
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<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Design</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/VP_design">Design</a></li> | ||
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human Practice</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human Practice</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Attribution</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Attribution</a></li> | ||
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<ul> | <ul> | ||
| - | <li><a class="arrow | + | <li><a onClick="out('cm-nav')" class="arrow">Protocols</a> |
<ul> | <ul> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li> | ||
Latest revision as of 13:40, 5 October 2011
Protocols
Gas chromatography (GC) Protocol
Method:Culture samples were centrifuged (12500×g, 25°C, 5 min), and the supernatants were analyzed for isobutanol. Isobutanol products were analyzed by GC equipped with flame ionization detector(FID) and a 50-m Ultra-2 column (Hewlett Packard, USA). The separation of alcohol compounds was carried out with a EQUity capirally column (SUPELCO, 60 m; 0.32 mm inside diameter; 1.0 μm film thickness). Filtrated samples were injected in split injection mode (1:10 split ratio).Nitrogen (constant flow 1 mL/min) was used as a carrier gas. The temperature of the injector was 150 °C. The oven program was as follows: 40°C ramp to 100 °C at 20 °C/min, 100 °C for 1 min, ramp to 130 °C at 15 °C/min, 130°C for 4 min.
