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Team:HKU-Hong Kong/Results
From 2011.igem.org
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|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;"|Results | |style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;"|Results | ||
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| - | |style="width:900px;font-size: 1. | + | |style="width:900px;font-size:1.7em;"|'''GFP intensity''' |
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| - | Our team have successfully inserted the tetO2-GFP sites (tetO2-0 EGFP , tetO2-0 sfGFP and tetO2-1 EGFP) into the E.Coli MG1655 genome (attTn7 site) by recombination with our designed plasmid containing the tet-O2-GFP site. | + | |style="width:900px;"| |
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| + | Our team have successfully inserted the tetO2-GFP sites (tetO2-0 EGFP , tetO2-0 sfGFP and tetO2-1 EGFP) into the E.Coli MG1655 genome (attTn7 site) by recombination with our designed plasmid containing the tet-O2-GFP site. Subsequently, we transformed our silencer plasmid into these tetO2-GP strains and the GFP intensity is measured. | ||
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The GFP intensity results are as follow: | The GFP intensity results are as follow: | ||
The untransformed E.Coli MG1655 (tetO2-0 EGFP, tetO2-0 sfGFP or tetO2-1 EGFP incorporated into the genome) is set as control and the fluorescence intensity is calibrated as 100%. | The untransformed E.Coli MG1655 (tetO2-0 EGFP, tetO2-0 sfGFP or tetO2-1 EGFP incorporated into the genome) is set as control and the fluorescence intensity is calibrated as 100%. | ||
<LI>Note that the data collected are considered only when difference between values have the P-value is less than 0.05. | <LI>Note that the data collected are considered only when difference between values have the P-value is less than 0.05. | ||
Revision as of 12:34, 5 October 2011
| Results |
| GFP intensity |
|
Our team have successfully inserted the tetO2-GFP sites (tetO2-0 EGFP , tetO2-0 sfGFP and tetO2-1 EGFP) into the E.Coli MG1655 genome (attTn7 site) by recombination with our designed plasmid containing the tet-O2-GFP site. Subsequently, we transformed our silencer plasmid into these tetO2-GP strains and the GFP intensity is measured.
The untransformed E.Coli MG1655 (tetO2-0 EGFP, tetO2-0 sfGFP or tetO2-1 EGFP incorporated into the genome) is set as control and the fluorescence intensity is calibrated as 100%. |
