From 2011.igem.org

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Latest revision as of 05:35, 29 June 2011

Team: British Columbia - 2011.igem.org

Brainstorming

Week 1: Jun 5-11

Week 2: Jun 12-18

Week 3: Jun 19-25

Week 4: Jun 26-Jul 2

Week 5: Jul 3-9

Week 6: Jul 10-16

Week 7: Jul 17-23

Week 8: Jul 24-30

Week 9: Jul 31-Aug 6

Week 10: Aug 7-13

Week 11: Aug 14-20

Week 12: Aug 21-27

Week 13: Aug 28-Sep 3

Week 14: Sep 4-10

Week 15: Sep 11-17

Week 16: Sep 18-28

Americas Jamboree!!!

Week 19: Oct 9-15

Leading up to the Finals!

Week 2: Monday May 9 - Sunday May 15

Monday

Dr. Joanne Fox begins a week of iGEM laboratory training in AMBL, the teaching lab in Michael Smith Laboratories. The training sessions run from 10am-4pm; Joanne provided short lectures so everyone understands the magic that goes on behind the actual bench work. Today, those present learned about restriction digests, and then set up restriction digests of the insert and vector in the morning. In the afternoon, we learned about purification kits, CIP assays, and ligation.

Tuesday

Wednesday

Marianne and Vicki (returning team members) went over labeling etiquette, lab book organization, and the 3-antibiotic method in the morning. In the afternoon, the team returned to AMBL to conduct transformation experiments on Monday's ligated parts.

Thursday

We did plate analysis, and started a culture for plasmid preps. Not everyone had colonies on their plates, so some had to steal others' colonies to start the culture.

In the afternoon, the team met up to discuss the pine beetle project in more detail - the objectives, the future applications/implications, the parts we need, the wet lab, modeling, and human practices components.

Friday

We mini-prepped the plasmids.

@@ Line 1: / Line 1: @@
 {{Template:notebook}}
-Week 2: Monday May 2 - Friday May 6
+Week 2: Monday May 9 - Sunday May 15
-==Monday==
+=Monday=
-===
+Dr. Joanne Fox begins a week of iGEM laboratory training in AMBL, the teaching lab in Michael Smith Laboratories. The training sessions run from 10am-4pm; Joanne provided short lectures so everyone understands the magic that goes on behind the actual bench work. Today, those present learned about restriction digests, and then set up restriction digests of the insert and vector in the morning. In the afternoon, we learned about purification kits, CIP assays, and ligation.
-'''
-Monoterpene Synthase Project'''
-PsTPS-Pin:
+=Tuesday=
-     Objective: PCR with designed primers for Site Directed Mutagenesis following the Stratagene Quikchange Protocol.
-     Procedures: Following the protocol from Stratagene Quikchange Protocol
+=Wednesday=
-     Results: No PCR products were amplified after gel check with gel electrophoresis. Primers are visible on the gel.
+Marianne and Vicki (returning team members) went over labeling etiquette, lab book organization, and the 3-antibiotic method in the morning.
-              Possible degradation of the Pfu polymerase, which explains why the PCR has not worked
+In the afternoon, the team returned to AMBL to conduct transformation experiments on Monday's ligated parts.
+=Thursday=
+We did plate analysis, and started a culture for plasmid preps. Not everyone had colonies on their plates, so some had to steal others' colonies to start the culture.
+In the afternoon, the team met up to discuss the pine beetle project in more detail - the objectives, the future applications/implications, the parts we need, the wet lab, modeling, and human practices components.
+=Friday=
+We mini-prepped the plasmids.
+=Saturday=
+=Sunday=

Team:British Columbia/Week2