Team:TzuChiU Formosa/Humanpractice/workshop
From 2011.igem.org
(Difference between revisions)
Line 34: | Line 34: | ||
<br> | <br> | ||
<div align="right"> | <div align="right"> | ||
- | <font color="#000000" size= | + | <font color="#000000" size=4>Date: 9,10 September 2011<br> |
<br>Location: Tzu Chi University</font> | <br>Location: Tzu Chi University</font> | ||
</div> | </div> | ||
Line 40: | Line 40: | ||
<br> | <br> | ||
<font color="#228B22" size=6><b>S</b></font> | <font color="#228B22" size=6><b>S</b></font> | ||
- | <font color="#000000" size= | + | <font color="#000000" size=3>ummer is officially over and it’s the start of a brand new semester. In conjunction with the enrolment of the 100th year intake, we conducted a few workshops on the subject of synthetic biology in collaboration with the College of Life Sciences. Prof. Sun Der Shan and Prof. Woon Peng Yeong coordinated the workshops while our teammates operated as the tutors to the participants. Our team members not only carried out and learnt their project; they also shared their experiences with others and promote synthetic biology to the general public.</font> |
<br> | <br> | ||
<br><br><br><br> | <br><br><br><br> | ||
- | <font color="#000000" size= | + | <font color="#000000" size=3>During the 1st day of the workshops, we managed to cover various aspects of synthetic biology, from the introduction of synthetic biology including the history, current applications and future directions as well as the iGEM competition. On the other hand, we also performed the basic techniques in molecular biology such as plasmid isolation, enzyme digestion, Polymerase Chain Reaction, gel electrophoresis, insert preparation, gel extraction, ligation and transformation, cloning, autoclaving, flaming, primer design, growth curves, as well as screening techniques to the participants.</font> |
<br> | <br> | ||
<br> | <br> | ||
Line 50: | Line 50: | ||
<br> | <br> | ||
<br> | <br> | ||
- | <font color="#000000" size= | + | <font color="#000000" size=3>The highlight of the event is the plasmid isolation and enzyme digestion workshop held on the 2nd day. All the participants were given a chance to perform in the activity. We, as the tutors assisted them throughout the whole process. By the end of the workshop, they learned to cut a plasmid with restriction enzyme, to paste DNA with ligase and PCR amplification.</font> |
<br><br><br><br><br> | <br><br><br><br><br> | ||
Line 72: | Line 72: | ||
<br><font size=3>↑Ting-ting is assisting the participant to add TBE buffer</font> | <br><font size=3>↑Ting-ting is assisting the participant to add TBE buffer</font> | ||
<br> | <br> | ||
- | + | <br><br><br> | |
</html> | </html> |
Revision as of 20:17, 4 October 2011
Date: 9,10 September 2011
Location: Tzu Chi University
Location: Tzu Chi University
S ummer is officially over and it’s the start of a brand new semester. In conjunction with the enrolment of the 100th year intake, we conducted a few workshops on the subject of synthetic biology in collaboration with the College of Life Sciences. Prof. Sun Der Shan and Prof. Woon Peng Yeong coordinated the workshops while our teammates operated as the tutors to the participants. Our team members not only carried out and learnt their project; they also shared their experiences with others and promote synthetic biology to the general public.
During the 1st day of the workshops, we managed to cover various aspects of synthetic biology, from the introduction of synthetic biology including the history, current applications and future directions as well as the iGEM competition. On the other hand, we also performed the basic techniques in molecular biology such as plasmid isolation, enzyme digestion, Polymerase Chain Reaction, gel electrophoresis, insert preparation, gel extraction, ligation and transformation, cloning, autoclaving, flaming, primer design, growth curves, as well as screening techniques to the participants.
The highlight of the event is the plasmid isolation and enzyme digestion workshop held on the 2nd day. All the participants were given a chance to perform in the activity. We, as the tutors assisted them throughout the whole process. By the end of the workshop, they learned to cut a plasmid with restriction enzyme, to paste DNA with ligase and PCR amplification.
↑2nd day of the workshop wet-lab day:
↑Ting-ting is explaining to her how to use a pippette
↑Andrew is demonstrating the step in the fume hood
↑Plasmid isolation
↑Ting-ting is assisting the participant to add TBE buffer