Team:NYMU-Taipei/chassis

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(→Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...)
(→Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...)
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●Wolfe’s vitamin solution  10 ml(stock)
●Wolfe’s vitamin solution  10 ml(stock)
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|• MgSO<sub>4</sub>‧7H<sub>2</sub>O 3.0g
 
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• MnSO<sub>4</sub>‧H<sub>2</sub>O 0.5g
 
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• NaCl 1.0g
 
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• FeSO<sub>4</sub>‧7H<sub>2</sub>O 0.1g
 
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• CoCl<sub>3</sub>‧6H<sub>2</sub>O 0.1g
 
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• CaCl<sub>2</sub> 0.1g
 
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• ZnSO<sub>4</sub>‧7H<sub>2</sub>O 0.1g
 
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• CuSO<sub>4</sub>‧5H<sub>2</sub>O 0.01g
 
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• AlK(SO<sub>4</sub>)<sub>2</sub>‧12H<sub>2</sub>O 0.01g
 
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• H<sub>3</sub>BO<sub>3</sub> 0.01g
 
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• Na<sub>2</sub>Mo<sub>4</sub>‧2H<sub>2</sub>O 0.01g
 
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• Distilled water
 
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Revision as of 15:26, 4 October 2011

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Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...

  Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM).  In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.


MSGM's ingredients


(In grams per liter)

●succinic acid 0.74 g

●KH2PO4 0.68 g

●sodium nitrate 0.12 g

●sodium thioglycolate 0.1 g

●Wolfe’s vitamin solution 10 ml(stock)


●Wolfe’s mineral solution 5 ml(stock)

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