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Team:NYMU-Taipei/chassis
From 2011.igem.org
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(→Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...) |
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| - | |• | + | |• MgSO<sub>4</sub>‧7H<sub>2</sub>O 3.0g |
| - | • | + | • MnSO<sub>4</sub>‧H<sub>2</sub>O 0.5g |
• NaCl 1.0g | • NaCl 1.0g | ||
| - | • | + | • FeSO<sub>4</sub>‧7H<sub>2</sub>O 0.1g |
| - | • | + | • CoCl<sub>3</sub>‧6H<sub>2</sub>O 0.1g |
| - | • | + | • CaCl<sub>2</sub> 0.1g |
| - | • | + | • ZnSO<sub>4</sub>‧7H<sub>2<sub>O 0.1g |
| - | • | + | • CuSO<sub>4</sub>‧5H</sub>2<sub>O 0.01g |
| - | • | + | • AlK(SO<sub>4</sub>)<sub>2</sub>‧12H<sub>2</sub>O 0.01g |
| - | • | + | • H<sub>3</sub>BO<sub>3</sub> 0.01g |
| - | • | + | • Na<sub>2</sub>Mo<sub>4</sub>‧2H<sub>2</sub>O 0.01g |
• Distilled water | • Distilled water | ||
Revision as of 15:24, 4 October 2011
Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...
Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM). In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.
MSGM's ingredients
(In grams per liter)
●succinic acid 0.74 g
●KH2PO4 0.68 g
●sodium nitrate 0.12 g
●sodium thioglycolate 0.1 g
●Wolfe’s vitamin solution 10 ml(stock)
| • MgSO4‧7H2O 3.0g
• MnSO4‧H2O 0.5g • NaCl 1.0g • FeSO4‧7H2O 0.1g • CoCl3‧6H2O 0.1g • CaCl2 0.1g • ZnSO4‧7H2O 0.1g • CuSO4‧5H2O 0.01g • AlK(SO4)2‧12H2O 0.01g • H3BO3 0.01g • Na2Mo4‧2H2O 0.01g • Distilled water | |
●Wolfe’s mineral solution 5 ml(stock)
● ● ●
