Team:NYMU-Taipei/chassis

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(→Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...)
(→Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...)
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●Wolfe’s vitamin solution  10 ml(stock)
●Wolfe’s vitamin solution  10 ml(stock)
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●Wolfe’s mineral solution  5  ml(stock)
 
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{| border=1
{| border=1
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| rowspan=5 |  [[image:NYMU_Chchang.jpg]]
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| Dr. Chuan-Hsiung Chang (張傳雄)
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|• MgSO4. 7H2O 3.0g
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• MnSO4. H2O 0.5g
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• NaCl 1.0g
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• FeSO4. 7H2O 0.1g
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• CoCl3 6H2O 0.1g
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• CaCl2 0.1g
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• ZnSO4. 7H2O 0.1g
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• CuSO4. 5 H2O 0.01g
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• ALK(SO4)2 12H2O 0.01g
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• H3BO3 0.01g
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• Na2MO4 2H2O 0.01g
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• Distilled water
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| Assistant professor, Center for Systems and Synthetic Biology, Institute of Biomedical Informatics, National Yang Ming University
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| Director of Bioinformatics Program
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| Office phone number: 02-2826-7316, Cell phone: 0918036745
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| Email: cchang@ym.edu.tw
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●Wolfe’s mineral solution  5  ml(stock)
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Revision as of 15:20, 4 October 2011

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Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...

  Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM).  In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.


MSGM's ingredients


(In grams per liter)

●succinic acid 0.74 g

●KH2PO4 0.68 g

●sodium nitrate 0.12 g

●sodium thioglycolate 0.1 g

●Wolfe’s vitamin solution 10 ml(stock)

• MgSO4. 7H2O 3.0g

• MnSO4. H2O 0.5g • NaCl 1.0g • FeSO4. 7H2O 0.1g • CoCl3 6H2O 0.1g • CaCl2 0.1g • ZnSO4. 7H2O 0.1g • CuSO4. 5 H2O 0.01g • ALK(SO4)2 12H2O 0.01g • H3BO3 0.01g • Na2MO4 2H2O 0.01g • Distilled water


●Wolfe’s mineral solution 5 ml(stock)

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