Team:ZJU-China/Notebook/September

From 2011.igem.org

(Difference between revisions)
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</table>
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<p>&nbsp</p>
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<p>&nbsp;</p>
</div>
</div>
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<div class="block" id="nsheet">
 +
<h3>Week11</h3><hr/>
 +
 +
<table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">
 +
  <tr><td width="76">Day</td><td width="349">Note</td></tr>
 +
 
 +
  <tr>
 +
    <td id="sheetleft">Sept.12th
 +
Monday
 +
 +
 +
</td>
 +
    <td><table id="intable" width="603" border="0" cellspacing="0" cellpadding="1">
 +
 
 +
    <td width="191">• Colony PCR: 1A3, 1K3, lig RFP+YFP+1C3.
 +
 +
</td>
 +
 
 +
 
 +
    <td  >• Run the PCR results.
 +
 +
</td>
 +
<td>• Miniprep: lig RFP+YFP+1C3
 +
</td>
 +
 
 +
</table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.13th
 +
Tuesday
 +
 +
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1">
 +
  <td>• Transform 2M, 2I, 6I, 23L.</td>
 +
  <td>• Purification: 1K3</td>
 +
 +
</table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.14th
 +
Wednesday
 +
 +
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="640" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
  <td>• Miniprep: 20H, 20J, 22B</td>
 +
  <td>• Cut: 1K3 with E+P, lig3 with S+E, 12I(CFP) with P+X,</td>
 +
  <td>• Gel excision and /or purification: 1K3, lig3, 12I(CFP)</td>
 +
  <td>• Run the gel.<br/>
 +
• Culture: 1I, 1K, 3L, 5E, 7C
 +
</td>
 +
<td>• Miniprep: RFP+YFP+1C3.<br/>
 +
• Run the gel of 1K3, lig3, CFP again.
 +
</td>
 +
<td>• Gel excision and purification<br/>
 +
• Ligation: Lig3+CFP+1K3
 +
</td>
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.15th
 +
Thursday
 +
 +
 +
 +
</td>
 +
    <td><table width="618" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
 
 +
<td>• Preserve: 1I, 1K, 3C, 5E, 7C<br/>
 +
• Transform the ligation results
 +
</td>
 +
<td>• Colony PCR: 2M, 2I，6I，23L<br/>
 +
• Cut: 20H, 20J, 22B with X+P
 +
 +
</td>
 +
<td>• Miniprep: 2M-1, 2I-1, 6I-1, 23L-1<br/>
 +
• Cut: 2M, 2I with E+S
 +
 +
 +
</td>
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.16th
 +
Friday
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="627" border="0" cellspacing="0" cellpadding="1">
 +
      <tr>
 +
      <td >• Colony PCR: RFP+YFP+1K3</td>
 +
      <td>• Cut 13K with E+S, 10I with P+X, 1C3 with E+P
 +
</td>
 +
<td>• Run the gel: 13K, 10I, 1C3, 2I, 2M, 20J, 22H, 22B</td>
 +
<td>• Ligation: 13K+10I+1C3<br/>
 +
• Culture: YFP(Amp), CFP(Amp)
 +
</td>
 +
      </tr>
 +
    </table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.17th
 +
Saturday
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="620" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
    <td>• Order primers for 20H, 20J<br/>
 +
• Culture 20H-1, 20J-1, 22B-1
 +
</td>
 +
  <td>• Plate the ligation results<br/>
 +
• Culture RFP+YFP+CFP+1K3
 +
 +
</td>
 +
<td>• Colony PCR: 3A, 1C3<br/>
 +
• Preserve: 1K3, RFP+YFP+1C3.
 +
 +
</td>
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.18th
 +
Sunday
 +
 +
 +
</td>
 +
    <td><table width="620" border="0" cellspacing="0" cellpadding="1">
 +
      <tr>
 +
        <td width="244">• Miniprep: 20J, 20H, RFP+YFP+CFP+1K3
 +
1A3
 +
</td>
 +
<td width="164">• Run the gel of PCR results
 +
 +
</td>
 +
<td>• Cut RFP+YFP+CFP+1K3
 +
with E+P<br/>
 +
• Colony PCR: 13K+10I+1C3
 +
</td>
 +
<td>• PCR: fdhF+rush+Vgb+tetR<br/>
 +
• Preserve: 20H, 20J, 22B
 +
</td>
 +
<td>• Run the gel: 20H, 20J, 22B<br/>
 +
• Cut the bands and purification.
 +
</td>
 +
<td>• Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3<br/>
 +
• Culture: 13K+10I+1C3<br/>
 +
• Grad PCR: fdhF.
 +
</td>
 +
      </tr>
 +
    </table></td>
 +
  </tr>
 +
 +
</table>
 +
<p>&nbsp;</p>
 +
</div>
 +
 +
<div class="block" id="nsheet">
 +
<h3>Week12</h3><hr/>
 +
 +
<table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">
 +
  <tr><td width="76">Day</td><td width="349">Note</td></tr>
 +
 
 +
  <tr>
 +
    <td id="sheetleft">Sept.19th
 +
Monday
 +
 +
 +
 +
</td>
 +
    <td><table id="intable" width="603" border="0" cellspacing="0" cellpadding="1">
 +
 
 +
    <td width="191">• Miniprep: 13K+10I+1C3, 1C3<br/>
 +
• Run the gel of digestion results.
 +
 +
</td>
 +
 
 +
 
 +
    <td  >• Cut: 13K+10I+1C3, 1A3, fdhF
 +
 +
</td>
 +
<td>• Run the gel: 20H, 20J, rush+vgb, RYC+1K3, fdhF, 13K+10I, 1A3
 +
</td>
 +
  <td>• Cut and purification: fdhF, 13K+10I, 1A3<br/>
 +
• Culture: CFP, YFP
 +
</td>
 +
<td>• Purification: Rush PCR<br/>
 +
• Cut: 22B with X, rush PCR results with S
 +
</td>
 +
 +
</table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.20th
 +
Tuesday
 +
 +
 +
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1">
 +
  <td>• Run the 20H, 20J from PCR results.<br/>
 +
• Transform: fdhF+13K+10I+1A3
 +
</td>
 +
  <td>• Purification: 20B, Rush<br/>
 +
• Ligation: 22B+Rush
 +
</td>
 +
<td>• Purification: ligation results<br/>
 +
• Cut: the ligation results with X+S
 +
</td>
 +
<td>• PCR 20H<br/>
 +
• Self-ligation: rush+22M
 +
</td>
 +
</table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.21st
 +
Wednesday
 +
 +
 +
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="640" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
  <td>• Colony PCR: fdhF+13K+10I+1A3 on plates</td>
 +
  <td>• Run the gel of PCR results</td>
 +
  <td>• Culture the positive results.</td>
 +
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.22nd
 +
Thursday
 +
 +
 +
 +
</td>
 +
    <td><table width="618" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
 
 +
<td>• Miniprep: fdhF+13K+10I+1A3<br/>
 +
• Preserve the above.
 +
 +
</td>
 +
<td>• Cut and run the results:
 +
fdhF+13K+10I+1A3, 13K+10I+1C3
 +
 +
 +
</td>
 +
<td>• Cut: RFP with E+S, YFP with X+P, 1K3 with E+P, 1C3 with E+P
 +
</td>
 +
<td>• Purification the digestion results.</td>
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.23rd
 +
Friday
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="627" border="0" cellspacing="0" cellpadding="1">
 +
      <tr>
 +
      <td >• Ligate: RFP+1A3/ YFP+1C3/1K3, RFP+1A3/1C3</td>
 +
      <td>• Transform the ligation results.
 +
</td>
 +
 +
      </tr>
 +
    </table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept. 24th
 +
Saturday
 +
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="620" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
    <td>• Order primers for 20H, 20J<br/>
 +
• Culture 20H-1, 20J-1, 22B-1
 +
</td>
 +
  <td>• Plate the ligation results<br/>
 +
• Culture RFP+YFP+CFP+1K3
 +
 +
</td>
 +
<td>• Colony PCR: 3A, 1C3<br/>
 +
• Preserve: 1K3, RFP+YFP+1C3.
 +
 +
</td>
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.25th
 +
Sunday
 +
 +
</td>
 +
    <td><table width="620" border="0" cellspacing="0" cellpadding="1">
 +
      <tr>
 +
        <td width="244">• Miniprep: 20J, 20H, RFP+YFP+CFP+1K3
 +
1A3
 +
</td>
 +
<td width="164">• Run the gel of PCR results
 +
 +
</td>
 +
<td>• Cut RFP+YFP+CFP+1K3
 +
with E+P<br/>
 +
• Colony PCR: 13K+10I+1C3
 +
</td>
 +
<td>• PCR: fdhF+rush+Vgb+tetR<br/>
 +
• Preserve: 20H, 20J, 22B
 +
</td>
 +
<td>• Run the gel: 20H, 20J, 22B<br/>
 +
• Cut the bands and purification.
 +
</td>
 +
<td>• Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3<br/>
 +
• Culture: 13K+10I+1C3<br/>
 +
• Grad PCR: fdhF.
 +
</td>
 +
      </tr>
 +
    </table></td>
 +
  </tr>
 +
 +
</table>
 +
<p>&nbsp;</p>
 +
</div>
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Revision as of 06:43, 3 October 2011

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Labnote

 Biobrick group | Biofilm group 

This group...........

Week9

DayNote
Aug.29th Monday
• Meeting. Summarize all the experiment work so far. • Cut: 22M(X+P), 1C3(E+P)
Aug.30th Tuesday
Aug.31st Wednesday
• Cut vgb+YFP+tetR, fdhF+RFP+tetR.
• Run the digestion results		 • Culture the positive results
Sept.1st Thursday
• Miniprep: Y#4, R#2/3/4/5
• Cut Y#4, R#2/3/4/5 		•Run the gel.
• Send R#3,Y#4 sample to sequencing 		• Hypoxia culture: RFP3, RFP4
• Preserve Y#4, R#2/3/4/5
Sept.2nd Friday
• Phusion PCR • Hypoxia Culture: R, Y
• Run the PCR results. Bands are confirmed right. 		• Purification: vgb, YFP, tetR.
Sept.3rd Saturday
• Culture: YFP, RFP, A25
• Culture in micro-oxygen: YFP, RFP, A25 		• Culture in slope medium: RFP, YFP, A25
• Hypoxia culture: RFP, YFP, A25 		• Check the fluorescence.
• Neither RFP in hypoxia or oxygen condition. Little YFP both in hypoxia and oxygen.
Sept.4th Sunday
• Miniprep: RFP3, YFP4 • Cut: RFP3, YFP4, !C3.

 

Week10

DayNote
Sept.5th Monday
• Run the gel: Yu’s PCR results, 1C3, RFP3, YFP4
• Purification: RFP3, YFP4 		• Ligation: RFP3+1C3, YFP4+1C3
• Hypoxia culture: RFP3, YFP4, A25. 		• Transform the ligation results.
• Confirmed RFP expression only in hypoxia condition.
Sept.6th Tuesday
• Check the RFP, YFP plates. • Colony PCR
Sept.7th Wednesday
• Culture YFP1/2, RFP3/4
Sept.8th Thurday
• Preserve the strain of RFP1, YFP1/2 in 1C3.
• Miniprep the remains. 		• Cut: YFP1/2, RFP1 • Run the digestion results.
• Culture RFP2
Sept.9th Friday
• Miniprep: RFP2, YFP2, RFP1 • Cut them and run the gel.
Sept.10th Saturday
• Cut YFP(1A3), RFP(1A3) with S+E • Purification: RFP(1A3), YFP(1A3) • Ligation: RFP+1C3, YFP+1C3 • Transform the ligation results.
Sept.11th Sunday
• Check the plates. • Culture lig RFY+YFP+IC3
• Culture 1K3 		• Colony PCR: RFP(1C3)， YFP(1C3)

 

Week11

DayNote
Sept.12th Monday
• Colony PCR: 1A3, 1K3, lig RFP+YFP+1C3. • Run the PCR results. • Miniprep: lig RFP+YFP+1C3
Sept.13th Tuesday
• Transform 2M, 2I, 6I, 23L. • Purification: 1K3
Sept.14th Wednesday
• Miniprep: 20H, 20J, 22B • Cut: 1K3 with E+P, lig3 with S+E, 12I(CFP) with P+X, • Gel excision and /or purification: 1K3, lig3, 12I(CFP) • Run the gel.
• Culture: 1I, 1K, 3L, 5E, 7C 		• Miniprep: RFP+YFP+1C3.
• Run the gel of 1K3, lig3, CFP again. 		• Gel excision and purification
• Ligation: Lig3+CFP+1K3
Sept.15th Thursday
• Preserve: 1I, 1K, 3C, 5E, 7C
• Transform the ligation results 		• Colony PCR: 2M, 2I，6I，23L
• Cut: 20H, 20J, 22B with X+P 		• Miniprep: 2M-1, 2I-1, 6I-1, 23L-1
• Cut: 2M, 2I with E+S
Sept.16th Friday
• Colony PCR: RFP+YFP+1K3 • Cut 13K with E+S, 10I with P+X, 1C3 with E+P • Run the gel: 13K, 10I, 1C3, 2I, 2M, 20J, 22H, 22B • Ligation: 13K+10I+1C3
• Culture: YFP(Amp), CFP(Amp)
Sept.17th Saturday
• Order primers for 20H, 20J
• Culture 20H-1, 20J-1, 22B-1 		• Plate the ligation results
• Culture RFP+YFP+CFP+1K3 		• Colony PCR: 3A, 1C3
• Preserve: 1K3, RFP+YFP+1C3.
Sept.18th Sunday
• Miniprep: 20J, 20H, RFP+YFP+CFP+1K3 1A3 • Run the gel of PCR results • Cut RFP+YFP+CFP+1K3 with E+P
• Colony PCR: 13K+10I+1C3 		• PCR: fdhF+rush+Vgb+tetR
• Preserve: 20H, 20J, 22B 		• Run the gel: 20H, 20J, 22B
• Cut the bands and purification. 		• Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3
• Culture: 13K+10I+1C3
• Grad PCR: fdhF.

 

Week12

DayNote
Sept.19th Monday
• Miniprep: 13K+10I+1C3, 1C3
• Run the gel of digestion results. 		• Cut: 13K+10I+1C3, 1A3, fdhF • Run the gel: 20H, 20J, rush+vgb, RYC+1K3, fdhF, 13K+10I, 1A3 • Cut and purification: fdhF, 13K+10I, 1A3
• Culture: CFP, YFP 		• Purification: Rush PCR
• Cut: 22B with X, rush PCR results with S
Sept.20th Tuesday
• Run the 20H, 20J from PCR results.
• Transform: fdhF+13K+10I+1A3 		• Purification: 20B, Rush
• Ligation: 22B+Rush 		• Purification: ligation results
• Cut: the ligation results with X+S 		• PCR 20H
• Self-ligation: rush+22M
Sept.21st Wednesday
• Colony PCR: fdhF+13K+10I+1A3 on plates • Run the gel of PCR results • Culture the positive results.
Sept.22nd Thursday
• Miniprep: fdhF+13K+10I+1A3
• Preserve the above. 		• Cut and run the results: fdhF+13K+10I+1A3, 13K+10I+1C3 • Cut: RFP with E+S, YFP with X+P, 1K3 with E+P, 1C3 with E+P • Purification the digestion results.
Sept.23rd Friday
• Ligate: RFP+1A3/ YFP+1C3/1K3, RFP+1A3/1C3 • Transform the ligation results.
Sept. 24th Saturday
• Order primers for 20H, 20J
• Culture 20H-1, 20J-1, 22B-1 		• Plate the ligation results
• Culture RFP+YFP+CFP+1K3 		• Colony PCR: 3A, 1C3
• Preserve: 1K3, RFP+YFP+1C3.
Sept.25th Sunday
• Miniprep: 20J, 20H, RFP+YFP+CFP+1K3 1A3 • Run the gel of PCR results • Cut RFP+YFP+CFP+1K3 with E+P
• Colony PCR: 13K+10I+1C3 		• PCR: fdhF+rush+Vgb+tetR
• Preserve: 20H, 20J, 22B 		• Run the gel: 20H, 20J, 22B
• Cut the bands and purification. 		• Run the gel: 13K+10I+1C3, RFP+YFP+CFP+1K3
• Culture: 13K+10I+1C3
• Grad PCR: fdhF.

 

Retrieved from "http://2011.igem.org/Team:ZJU-China/Notebook/September"