Team:ZJU-China/Notebook/September

From 2011.igem.org

(Difference between revisions)
Line 267: Line 267:
<p>&nbsp;</p>  
<p>&nbsp;</p>  
</div>  
</div>  
 +
 +
<div class="block" id="nsheet">
 +
<h3>Week10</h3><hr/>
 +
<table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">
 +
  <tr><td width="76">Day</td><td width="349">Note</td></tr>
 +
 
 +
  <tr>
 +
    <td id="sheetleft">Sept.5th
 +
Monday
 +
 +
</td>
 +
    <td><table id="intable" width="603" border="0" cellspacing="0" cellpadding="1">
 +
 
 +
    <td width="191">• Run the gel: Yu’s PCR results, 1C3, RFP3, YFP4<br/>
 +
• Purification: RFP3, YFP4
 +
 +
</td>
 +
 
 +
 
 +
    <td  >• Ligation: RFP3+1C3, YFP4+1C3<br/>
 +
• Hypoxia culture: RFP3, YFP4, A25.
 +
 +
</td>
 +
<td>• Transform the ligation results.<br/>
 +
• Confirmed RFP expression only in hypoxia condition.
 +
</td>
 +
 
 +
</table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.6th
 +
Tuesday
 +
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1">
 +
  <td>• Check the RFP, YFP plates.</td>
 +
  <td>• Colony PCR</td>
 +
 +
</table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.7th
 +
Wednesday
 +
 +
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="640" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
  <td>• Culture YFP1/2, RFP3/4</td>
 +
 
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.8th
 +
Thurday
 +
 +
 +
</td>
 +
    <td><table width="618" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
 
 +
<td>• Preserve the strain of RFP1, YFP1/2 in 1C3.<br/>
 +
• Miniprep the remains.
 +
 +
</td>
 +
<td>• Cut: YFP1/2, RFP1
 +
</td>
 +
<td>• Run the digestion results.<br/>
 +
• Culture RFP2
 +
 +
</td>
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.9th
 +
Friday
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="627" border="0" cellspacing="0" cellpadding="1">
 +
      <tr>
 +
      <td >• Miniprep: RFP2, YFP2, RFP1</td>
 +
      <td>• Cut them and run the gel.
 +
</td>
 +
 +
      </tr>
 +
    </table></td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.10th
 +
Saturday
 +
 +
 +
 +
 +
</td>
 +
    <td><table width="620" border="0" cellspacing="0" cellpadding="1">
 +
  <tr>
 +
    <td>• Cut YFP(1A3), RFP(1A3) with S+E
 +
 +
 +
</td>
 +
  <td>• Purification: RFP(1A3), YFP(1A3)
 +
</td>
 +
<td>• Ligation: RFP+1C3, YFP+1C3
 +
</td>
 +
<td>• Transform the ligation results.</td>
 +
  </tr>
 +
</table>
 +
</td>
 +
  </tr>
 +
  <tr>
 +
    <td id="sheetleft">Sept.11th
 +
Sunday
 +
 +
 +
 +
</td>
 +
    <td><table width="620" border="0" cellspacing="0" cellpadding="1">
 +
      <tr>
 +
        <td width="244">• Check the plates.
 +
 +
</td>
 +
<td width="164">• Culture lig RFY+YFP+IC3<br/>
 +
• Culture 1K3
 +
 +
</td>
 +
<td>• Colony PCR: RFP(1C3)， YFP(1C3)</td>
 +
      </tr>
 +
    </table></td>
 +
  </tr>
 +
 +
</table>
 +
<p>&nbsp</p>
 +
</div>
 +
<script type="text/javascript">
<script type="text/javascript">

Revision as of 06:41, 3 October 2011

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">

Labnote

 Biobrick group | Biofilm group 

This group...........

Week9

DayNote
Aug.29th Monday
• Meeting. Summarize all the experiment work so far. • Cut: 22M(X+P), 1C3(E+P)
Aug.30th Tuesday
Aug.31st Wednesday
• Cut vgb+YFP+tetR, fdhF+RFP+tetR.
• Run the digestion results		 • Culture the positive results
Sept.1st Thursday
• Miniprep: Y#4, R#2/3/4/5
• Cut Y#4, R#2/3/4/5 		•Run the gel.
• Send R#3,Y#4 sample to sequencing 		• Hypoxia culture: RFP3, RFP4
• Preserve Y#4, R#2/3/4/5
Sept.2nd Friday
• Phusion PCR • Hypoxia Culture: R, Y
• Run the PCR results. Bands are confirmed right. 		• Purification: vgb, YFP, tetR.
Sept.3rd Saturday
• Culture: YFP, RFP, A25
• Culture in micro-oxygen: YFP, RFP, A25 		• Culture in slope medium: RFP, YFP, A25
• Hypoxia culture: RFP, YFP, A25 		• Check the fluorescence.
• Neither RFP in hypoxia or oxygen condition. Little YFP both in hypoxia and oxygen.
Sept.4th Sunday
• Miniprep: RFP3, YFP4 • Cut: RFP3, YFP4, !C3.

 

Week10

DayNote
Sept.5th Monday
• Run the gel: Yu’s PCR results, 1C3, RFP3, YFP4
• Purification: RFP3, YFP4 		• Ligation: RFP3+1C3, YFP4+1C3
• Hypoxia culture: RFP3, YFP4, A25. 		• Transform the ligation results.
• Confirmed RFP expression only in hypoxia condition.
Sept.6th Tuesday
• Check the RFP, YFP plates. • Colony PCR
Sept.7th Wednesday
• Culture YFP1/2, RFP3/4
Sept.8th Thurday
• Preserve the strain of RFP1, YFP1/2 in 1C3.
• Miniprep the remains. 		• Cut: YFP1/2, RFP1 • Run the digestion results.
• Culture RFP2
Sept.9th Friday
• Miniprep: RFP2, YFP2, RFP1 • Cut them and run the gel.
Sept.10th Saturday
• Cut YFP(1A3), RFP(1A3) with S+E • Purification: RFP(1A3), YFP(1A3) • Ligation: RFP+1C3, YFP+1C3 • Transform the ligation results.
Sept.11th Sunday
• Check the plates. • Culture lig RFY+YFP+IC3
• Culture 1K3 		• Colony PCR: RFP(1C3)， YFP(1C3)

&nbsp

Retrieved from "http://2011.igem.org/Team:ZJU-China/Notebook/September"