Team:ZJU-China/Notebook/September

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Labnote

Biobrick group | Biofilm group

This group...........

May&June

In the two months we discussed some new ideas and finally decided our project
>> Click to see our brainstorm

July

In this month we......
>>Click to see

August

In this month we......
>>Click to see

September

In this month we......
>>Click to see

Week9

Day

Note

Aug.29th Monday

• Meeting. Summarize all the experiment work so far.

• Cut: 22M(X+P), 1C3(E+P)

Aug.30th Tuesday

Aug.31st Wednesday

• Cut vgb+YFP+tetR, fdhF+RFP+tetR.
• Run the digestion results

• Culture the positive results

Sept.1st Thursday

• Miniprep: Y#4, R#2/3/4/5
• Cut Y#4, R#2/3/4/5

•Run the gel.
• Send R#3,Y#4 sample to sequencing

• Hypoxia culture: RFP3, RFP4
• Preserve Y#4, R#2/3/4/5

Sept.2nd Friday

• Phusion PCR

• Hypoxia Culture: R, Y
• Run the PCR results. Bands are confirmed right.

• Purification: vgb, YFP, tetR.

Sept.3rd Saturday

• Culture: YFP, RFP, A25
• Culture in micro-oxygen: YFP, RFP, A25

• Culture in slope medium: RFP, YFP, A25
• Hypoxia culture: RFP, YFP, A25

• Check the fluorescence.
• Neither RFP in hypoxia or oxygen condition. Little YFP both in hypoxia and oxygen.

Sept.4th Sunday

• Miniprep: RFP3, YFP4

• Cut: RFP3, YFP4, !C3.

@@ Line 123: / Line 123: @@
    <div class="bigblock">
 <div class="block" id="nsheet">
-<h3>Week1</h3><hr/>
+<h3>Week9</h3><hr/>
 <table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">
    <tr><td width="76">Day</td><td width="349">Note</td></tr>
    <tr>
-     <td id="sheetleft">Jul.4th Monday</td>
+     <td id="sheetleft">Aug.29th
-     <td><table id="intable" width="328" border="0" cellspacing="0" cellpadding="1">
+Monday
+</td>
+     <td><table id="intable" width="603" border="0" cellspacing="0" cellpadding="1">
-     <td width="128">• Aerobic cultivation of DH5α</td>
+     <td width="191">• Meeting. Summarize all the experiment work so far.
+</td>
-     <td width="196">• Preparation of apparatus for the formation of biofilm</td>
+     <td width="249" >• Cut: 22M(X+P), 1C3(E+P)
+</td>
 </table></td>
    </tr>
    <tr>
-     <td id="sheetleft">Jul 5th Tuesday
+     <td id="sheetleft">Aug.30th
-</td>
+Tuesday
-     <td>&nbsp;</td>
-   </tr>
-   <tr>
-     <td id="sheetleft">Jul.6th
+</td>
+     <td><table id="intable" width="615" border="0" cellspacing="0" cellpadding="1">
+</table></td>
+   </tr>
+   <tr>
+     <td id="sheetleft">Aug.31st
 Wednesday
-</td>
-     <td><table width="217" border="0" cellspacing="0" cellpadding="1">
-   <tr>
-    <td width="215">•Receiving  primers ordered previously</td>
-   </tr>
+</td>
-</table>
+     <td><table width="640" border="0" cellspacing="0" cellpadding="1">
-</td>
+   <tr>
-   </tr>
+   <td>• Cut vgb+YFP+tetR, fdhF+RFP+tetR.<br/>• Run the digestion results</td>
-   <tr>
+   <td>• Culture the positive results</td>
-     <td id="sheetleft">Jul.7th
+   </tr>
+</table>
+</td>
+   </tr>
+   <tr>
+     <td id="sheetleft">Sept.1st
 Thursday
-</td>
-     <td><table width="238" border="0" cellspacing="0" cellpadding="1">
-   <tr>
-    <td width="200">• Preparation of the aliquot of the primers</td>
-    <td width="200">• Something wrong with a shaking incubator</td>
-   </tr>
+</td>
-</table>
+     <td><table width="618" border="0" cellspacing="0" cellpadding="1">
-</td>
+   <tr>
-   </tr>
-   <tr>
+<td>• Miniprep: Y#4, R#2/3/4/5<br/>
-     <td id="sheetleft">Jul.8th
+• Cut Y#4, R#2/3/4/5
+</td>
+<td>•Run the gel.<br/>
+• Send R#3,Y#4 sample to sequencing
+</td>
+<td>• Hypoxia culture: RFP3, RFP4<br/>
+• Preserve Y#4, R#2/3/4/5
+</td>
+   </tr>
+</table>
+</td>
+   </tr>
+   <tr>
+     <td id="sheetleft">Sept.2nd
 Friday
-</td>
-     <td><table width="222" border="0" cellspacing="0" cellpadding="1">
-       <tr>
-        <td width="155">• Preparation of culture plates for the transformations</td>
+</td>
-       </tr>
+     <td><table width="627" border="0" cellspacing="0" cellpadding="1">
-     </table></td>
+       <tr>
-   </tr>
+       <td >• Phusion PCR</td>
-   <tr>
+       <td>• Hypoxia Culture: R, Y<br/>
-     <td id="sheetleft">Jul.9th
+• Run the PCR results. Bands are confirmed right.
+</td>
+<td>• Purification: vgb, YFP, tetR.</td>
+       </tr>
+     </table></td>
+   </tr>
+   <tr>
+     <td id="sheetleft">Sept.3rd
 Saturday
-</td>
-     <td><table width="313" border="0" cellspacing="0" cellpadding="1">
-   <tr>
-     <td width="127">• Preparation of culture plates for the transformations</td>
+</td>
-    <td width="182">• protocols of transformation</td>
+     <td><table width="620" border="0" cellspacing="0" cellpadding="1">
-   </tr>
+   <tr>
-</table>
+     <td>• Culture: YFP, RFP, A25<br/>
-</td>
+• Culture in micro-oxygen: YFP, RFP, A25
-   </tr>
+</td>
-   <tr>
+  <td>• Culture in slope medium: RFP, YFP, A25<br/>
-     <td id="sheetleft">Jul.10th
+• Hypoxia culture: RFP, YFP, A25
+</td>
+<td>• Check the fluorescence.<br/>
+• Neither RFP in hypoxia or oxygen condition.
+Little YFP both in hypoxia and oxygen.
+</td>
+   </tr>
+</table>
+</td>
+   </tr>
+   <tr>
+     <td id="sheetleft">Sept.4th
 Sunday
-</td>
-     <td><table width="246" border="0" cellspacing="0" cellpadding="1">
-       <tr>
+</td>
-         <td>• Several colonies were picked up and cultivated in  5mL LB medium.
+     <td><table width="620" border="0" cellspacing="0" cellpadding="1">
-•Cryosectioning of biofilm
+       <tr>
-</td>
+         <td width="244">• Miniprep: RFP3, YFP4
-       </tr>
-     </table></td>
+</td>
-   </tr>
+<td width="164">• Cut: RFP3, YFP4, !C3.
+</td>
-</table>
+       </tr>
+     </table></td>
+   </tr>
+</table>
 <p>&nbsp;</p>
 </div>