Team:HKUST-Hong Kong/overview.html
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Revision as of 11:50, 2 October 2011
1. ASM
1.1. Theory – how to select?
The E. trojan is a synthetic E.coli strain that is engineered to lack an essential gene, nadE, in its genomic DNA. To survive, this strain has to rely on a pre- introduced plasmid (pDummy) bearing the essential gene; thus forcing the bacteria to maintain the plasmid until an alternative source of nadE gene is present. The pDummy, however, has been designed to have a temperature- sensitive origin of replication which would cease to function if the bacterial cells are incubated under higher incubation temperatures (>42ᵒC???). |
Overview & Background1 ASM 1.1 Theory – how to select? 1.2 Method of assembly 1.3 Component details Next on1.2 Method of assembly |
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