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Team:NCTU Formosa/RNA discussion
From 2011.igem.org
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol">Mutation</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_F">Flow</a></li> | + | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_F">Flow Cytometry</a></li> |
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_G">GC</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/protocol_G">GC</a></li> | ||
</ul> | </ul> | ||
Revision as of 09:50, 2 October 2011
RNA Thermometer
Discussion
Our experimental results indicate that high temperature decreased the translation rate of the target protein, and this temperature-dependent genetic circuit can control the expression level of the target protein by the host cell's incubation temperature. However, the translational activity of the BBa_K115002 at different temperatures cannot be quantified directly from experimental data. To overcome this problem, we provided a dynamic model which can quantitatively assess the translation strength of the BBa_K115002 at temperatures 27°C, 32°C, 37°C and 42°C.
