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Team:NCTU Formosa/RNA discussion
From 2011.igem.org
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<li><a href="https://2011.igem.org/Team:NCTU_Formosa/parts">Parts</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/parts">Parts</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/safty">Safety</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/safty">Safety</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/humanpractice">Human Practice</a></li> | ||
<li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Contributions</a></li> | <li><a href="https://2011.igem.org/Team:NCTU_Formosa/contributions">Contributions</a></li> | ||
<li><a class="arrow no-click">Notebook </a> | <li><a class="arrow no-click">Notebook </a> | ||
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</ul> | </ul> | ||
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| - | <div | + | <div id="blueBox"><p>RNA Thermometer</p></div> |
| - | <div | + | <div id="Box"><h2>Discussion</h2> |
| - | <p>Our experimental results indicate that high temperature decreased the translation rate of the target protein, and this temperature-dependent genetic circuit can control the expression level of the target protein by the host cell's incubation temperature. However, the translational activity of the | + | |
| + | <p> | ||
| + | Our experimental results indicate that high temperature decreased the translation rate of the target protein, and this temperature-dependent genetic circuit can control the expression level of the target protein by the host cell's incubation temperature. However, the translational activity of the <a href = "http://partsregistry.org/wiki/index.php?title=Part:BBa_K115002">BBa_K115002</a> at different temperatures cannot be quantified directly from experimental data. To overcome this problem, we provided a dynamic model which can quantitatively assess the translation strength of the <a href = "http://partsregistry.org/wiki/index.php?title=Part:BBa_K115002">BBa_K115002</a> at temperatures 27°C, 32°C, 37°C and 42°C. | ||
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Revision as of 09:33, 2 October 2011
RNA Thermometer
Discussion
Our experimental results indicate that high temperature decreased the translation rate of the target protein, and this temperature-dependent genetic circuit can control the expression level of the target protein by the host cell's incubation temperature. However, the translational activity of the BBa_K115002 at different temperatures cannot be quantified directly from experimental data. To overcome this problem, we provided a dynamic model which can quantitatively assess the translation strength of the BBa_K115002 at temperatures 27°C, 32°C, 37°C and 42°C.
