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| | == '''summary''' == | | == '''summary''' == |
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| - | ==June==
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.22 - 6.25|22]]
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.22 - 6.25|23]]
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.22 - 6.25|24]]
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.22 - 6.25|25]]
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.26 - 6.27|26]]
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.26 - 6.27|27]]
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.28|28]]
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.29|29]]
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| - | |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#6.30|30]]
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| - | |}
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| - | [<html><a href="#top">TOP</a></html>]
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| - | ===6.22 - 6.25===
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| - | Design primers.
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| - | ===6.26 - 6.27===
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| - | Culture streptomycin griseus strain, which is the source of afsA, arpA, padpA.
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| - | ===6.28===
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| - | afsA, arpA PCR using different annealing temperature, with gradient 4 ℃.
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| - | Identify them by 1% agarose gel electrophoresis.
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| - | Excise the gel slice and extract the fragments.
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| - | Second PCR using the products of gel extraction.
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| - | Electrophoresis PCR reaction system in 1.5% agarose gel.
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| - | Double digest the products of gel extraction by EcoR1-HF and Xba1.
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| - | Double digest the plasmid of B0015 by EcoR1-HF and Spe1.
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| - | Ligase afsA and arpA to the vector of B0015.
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| - | ===6.29===
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| - | Transform the ligation reaction system.
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| - | Sythesis the genes of CAI-1 system using DNAWorks assembly and PadpA PCR.
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| - | Identify them by 1% agarose gel electrophoresis.
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| - | Excise the gel slice of pqrr, J23106+B0034+luxU, PadpA and extract the fragments.
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| - | ligase the products of gel extraction to the vector pEASY-Blunt.
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| - | ===6.30===
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| - | Transform the ligation reaction system.
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| - | Pick six clones each plate and shave at 37℃ to amplify the bacteria.
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