|
|
Line 57: |
Line 57: |
| </div> | | </div> |
| | | |
- | <div id="before">
| + | |
- | <h2>Data for pre-existing parts</h2>
| + | |
- | <p>1. <a href="http://partsregistry.org/Part:BBa_I15010:Experience#User_Reviews">Experience</a> – <a href="http://partsregistry.org/Part:BBa_I15010 ">BBa_I15010</a> Cph8 (Cph1/EnvZ fusion) (UTAustin, iGEM2004):
| + | |
- | This part was used by 2011 WHU_China team as a part of light sensing system. However, the result of sequencing proves that this part in the 2011 kit turns out to be a vector without the fragment of cph8. 2011 WHU_China requested the same part from the 2011 kit of HUST_China, but the result of pcr and restriction enzymes cleavage shows that their plasmids have the same problem.</br>
| + | |
- | 2. <a href="http://partsregistry.org/Part:BBa_E0040:Experience#User_Reviews">Experience</a> – <a href="http://partsregistry.org/Part:BBa_E0040">BBa_E0040</a> Green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Endy Lab, iGEM2004):
| + | |
- | This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks.
| + | |
- | </p>
| + | |
- | </div>
| + | |
| | | |
| | | |
Data for our favorite part
BBa_K560000 Main page - Blue Light Sensing and Reporting System: This part shows the complete system of light sensing and reporting regulation in E.coli. Although it is not very stable, it can truly work in TrpR(-) E.coli.