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Team:Peking S/lab/notebook/fjc
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| + | {{Template:Http://2011.igem.org/Team:Peking S/box4notebook}} | ||
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| + | <font size=6> <font color="#FFFFFF">Yanrong Ma's Notebook</font></FONT> | ||
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== '''summary''' == | == '''summary''' == | ||
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| + | Cloned genes of A-factor synthase afsA and its receptor arpA,and I also standardized these two genes .Besides, I also constructed and characterized quorum sensing genes named cinI and cinR. I further characterized its feasibilities in constructing synthetic microbial consortia. | ||
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| + | =='''Contents'''== | ||
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| + | 6.24-7.5 | ||
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| + | Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion. | ||
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| + | |||
| + | 7.6-7.7 | ||
| + | |||
| + | |||
| + | Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing. | ||
| + | |||
| + | |||
| + | 7.8-7.12 | ||
| + | |||
| + | |||
| + | Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome. | ||
| + | |||
| + | |||
| + | 7.13-7.23 | ||
| + | |||
| + | |||
| + | Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters. | ||
| + | |||
| + | |||
| + | 7.24-8.13 | ||
| + | |||
| + | |||
| + | Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules. | ||
| + | |||
| + | |||
| + | 8.14-9.5 | ||
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| + | Testing A-factor system performance. | ||
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| + | 9.6-9.18 | ||
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| + | Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules. | ||
| + | |||
| + | |||
| + | 9.19-10.1 | ||
| + | |||
| + | |||
| + | Characterization of cinI/cinR system. | ||
Revision as of 06:35, 1 October 2011
Template:Https://2011.igem.org/Team:Peking S/bannerhidden
Yanrong Ma's Notebook
summary
Cloned genes of A-factor synthase afsA and its receptor arpA,and I also standardized these two genes .Besides, I also constructed and characterized quorum sensing genes named cinI and cinR. I further characterized its feasibilities in constructing synthetic microbial consortia.
Contents
6.24-7.5
Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion.
7.6-7.7
Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing.
7.8-7.12
Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome.
7.13-7.23
Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters.
7.24-8.13
Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules.
8.14-9.5
Testing A-factor system performance.
9.6-9.18
Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules.
9.19-10.1
Characterization of cinI/cinR system.
