Team:Peking S/lab/notebook/myr

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<font size=6> <font color="#FFFFFF">Yanrong Ma's Notebook</font></FONT>
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6.24-7.5
6.24-7.5
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Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion.
Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion.
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7.6-7.7
7.6-7.7
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Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing.
Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing.
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7.8-7.12
7.8-7.12
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Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome.
Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome.
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7.13-7.23
7.13-7.23
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Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters.  
Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters.  
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7.24-8.13
7.24-8.13
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Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules.
Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules.
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8.14-9.5
8.14-9.5
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Testing A-factor system performance.
Testing A-factor system performance.
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9.6-9.18
9.6-9.18
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Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules.
Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules.
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9.19-10.1
9.19-10.1
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Characterization of cinI/cinR system.
Characterization of cinI/cinR system.

Revision as of 04:49, 1 October 2011


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Yanrong Ma's Notebook

6.24-7.5

Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion.


7.6-7.7


Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing.


7.8-7.12


Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome.


7.13-7.23


Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters.


7.24-8.13


Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules.


8.14-9.5


Testing A-factor system performance.


9.6-9.18


Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules.


9.19-10.1


Characterization of cinI/cinR system.

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