Team:Duke/Notebook

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Latest revision as of 05:40, 29 September 2011

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling and Results	Notebook	Safety	Attributions

Notebook

Calendar
May, 2011
We began coming up with ideas. We first read several papers to decide what some weak points in synthetic biology were.

Early June, 2011
We decided that synthetic biology should start focusing on next generation techniques and application based network to gain popularity for the field.

June 15, 2011
We read several articles about zinc finger proteins as DNA binding domains.

June 16, 2011
Chose how to apply zinc fingers in SynBio. We originally meant to use combinatorial libraries of zinc finger proteins and put randomized orders of binding sites in bacterial DNA.

June 17-June 25
Idea is developed further: We chose to study zinc fingers as synthetic transcription factors and decide to use the bacterial 2 hybrid assay to characterize.

July 4-August 1
Complete B2H and realize that it isn’t as successful as we hoped.

August 5, 2011
Back to the drawing board: How can we make characterization. Got IT! apply CPEC to B2H assay construction to get results quicker.

August 7th, 2011
We design the toggle switch controller and spend the next two months focusing on making CPEC fragments for synthesis de novo. We also collected characterization data for ZFs through protein docking and made stochastic models for our network.

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-You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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-{|align="justify"
-|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
-|[[Image:Duke_logo.png|200px|right|frame]]
-|-
-|
-''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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+!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Duke Official Team Profile]
 !align="center"|[[Team:Duke/Project|Project]]
 !align="center"|[[Team:Duke/Parts|Parts Submitted to the Registry]]
-!align="center"|[[Team:Duke/Modeling|Modeling]]
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 !align="center"|[[Team:Duke/Notebook|Notebook]]
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 ==Notebook==
+<html>
-You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
+<body>
+<p>
+<b>Calendar</b><br/>
+May, 2011<br/>
+We began coming up with ideas. We first read several papers to decide what some weak points in synthetic biology were.<br/>
+<br/>
+Early June, 2011<br/>
+We decided that synthetic biology should start focusing on next generation techniques and application based network to gain popularity for the field.<br/>
+<br/>
+June 15, 2011<br/>
+We read several articles about zinc finger proteins as DNA binding domains.<br/>
+<br/>
+June 16, 2011<br/>
+Chose how to apply zinc fingers in SynBio. We originally meant to use combinatorial libraries of zinc finger proteins and put randomized orders of binding sites in bacterial DNA. <br/>
+<br/>
+June 17-June 25<br/>
+Idea is developed further: We chose to study zinc fingers as synthetic transcription factors and decide to use the bacterial 2 hybrid assay to characterize.<br/>
+<br/>
+July 4-August 1<br/>
+Complete B2H and realize that it isn’t as successful as we hoped.<br/>
+<br/>
+August 5, 2011<br/>
+Back to the drawing board: How can we make characterization. Got IT! apply CPEC to B2H assay construction to get results quicker.<br/>
+<br/>
+August 7th, 2011<br/>
+We design the toggle switch controller and spend the next two months focusing on making CPEC fragments for synthesis de novo. We also collected characterization data for ZFs through protein docking and made stochastic models for our network.<br/>
+</p>
+</body>
+</html>