Team:UT Dallas/data

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<h2>Data</h2>
<h2>Data</h2>
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<b>How our system works</b><br>
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<b>Data For Our Favorite New Parts</b><br>
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<img src="https://static.igem.org/mediawiki/2011/1/1b/Schematic.png" style="border 1px solid"><br><br>
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<br>PcstA-RBS-LuxI-terminator,  BBa_K569001 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569001">Main Page</a>): This part produces LuxI,an enzyme for creating acyl-homoserine lactones from normal cell metabolites, in the absence of glucose. This part is repressed by glucose.<br><br>
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<b>Data For Our Favorite New Parts</b>
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<br>PyeaR-ToxR-FGFR, BBa_K569013 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569013">Main Page</a>): PyeaR is a nitrate inducible promoter. ToxR+FGFR is a receptor fusion of two dimerizing proteins. FGFR responds to FGF by dimerizing; this causes ToxR to dimerize. When ToxR is dimerized it acts as a transcription factor for the ctx promoter. <br><br>
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<br>PcstA-RBS-LuxI-terminator,  BBa_K569001 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569001">Main Page</a>): This part produces LuxI,an enzyme for creating acyl-homoserine lactones from normal cell metabolites, in the absence of glucose. This part is repressed by glucose.<br>
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<br>PyeaR-ToxR-FGFR, BBa_K569013 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569013">Main Page</a>): PyeaR is a nitrate inducible promoter. ToxR+FGFR is a receptor fusion of two dimerizing proteins. FGFR responds to FGF by dimerizing; this causes ToxR to dimerize. When ToxR is dimerized it acts as a transcription factor for the ctx promoter. <br>
<br>SCP-ToxR-FGFR, BBa_K569014 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569014">Main Page</a>): This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.<br><br>
<br>SCP-ToxR-FGFR, BBa_K569014 (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K569014">Main Page</a>): This part constitutively produces the ToxR+FGFR fusion protein. ToxR+FGFR dimerizes in response to FGF and can then activate the ctx promoter from the bacteria Vibrio cholera. ToxR by itself is a transcription factor, while FGFR is a immune system sensor.<br><br>
<b>Data For Pre-existing Parts</b><br>
<b>Data For Pre-existing Parts</b><br>
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<a href="http://partsregistry.org/Part:BBa_K131010:Experience">Experience</a>
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We grew PcstA-RBS-LuxI-terminator and AHL-inducible ColicinE2-GFP transformed in BL21 E.coli cells. We combined the two cultures in various ratios and either added or did not add glucose (<a href="http://partsregistry.org/Part:BBa_K131010:Experience">experience</a>). We allowed these samples to continue growing for 2 hours and we took measurements using a fluorescent microscope.
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<br>We grew PcstA-RBS-LuxI-terminator and AHL-inducible ColicinE2-GFP transformed in BL21 E.coli cells. We combined the two cultures in various ratios and either added or did not add glucose. We allowed these samples to continue growing for 2 hours and we took measurements using a fluorescent microscope.
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Latest revision as of 05:33, 29 September 2011