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Team:Calgary/Notebook/Protocols/Process14
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<p> Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used. </p> | <p> Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used. </p> | ||
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<li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> | <li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> | ||
| - | <li> Add 10 μL of | + | <li> Add 10 μL of ddH<sub>2</sub>O (deionized water)</li> |
<li> Let the water incubate at room temperature for 10 minutes</li> | <li> Let the water incubate at room temperature for 10 minutes</li> | ||
| - | <li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies</li> | + | <li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.</li> |
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Latest revision as of 04:19, 29 September 2011
Notebook
Post-Regionals
Journal
- Defining Objectives
- Techniques Workshop
- Bioinformatics & qRT-PCR
- Sensory Element Fishing
- Electrochem - CPR Oxidation
- Electrochem - Reporter Gene
- Pseudomonas Tools
- Microalgae Tools
- NA Viability Assays
- May - July Group Journal
Protocols
Safety
Rehydration of Registry DNA
Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.
- Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
- Add 10 μL of ddH2O (deionized water)
- Let the water incubate at room temperature for 10 minutes
- Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.
