Team:Calgary/Notebook/Protocols/Process14

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      <td width="85%"><div class="heading"><a style="text-decoration:none" name="rehydration"></a> Rehydration</div></td>
 
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   <p> Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used. </p>
   <p> Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used. </p>
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     <li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li>
     <li> Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li>
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     <li> Add 10 μL of ddH20 (deionized water)</li>
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     <li> Add 10 μL of ddH<sub>2</sub>O (deionized water)</li>
     <li> Let the water incubate at room temperature for 10 minutes</li>
     <li> Let the water incubate at room temperature for 10 minutes</li>
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     <li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies</li>
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     <li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.</li>
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Latest revision as of 04:19, 29 September 2011


Rehydration of Registry DNA

Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.

  1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
  2. Add 10 μL of ddH2O (deionized water)
  3. Let the water incubate at room temperature for 10 minutes
  4. Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.














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