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 <div class="container">
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      <div style="filter:alpha(opacity=70);opacity:.7;">
-    <img src="https://static.igem.org/mediawiki/2011/2/20/Logotec.png" alt="ITESM" name="teclogo" width="146" height="52" id="teclogo">
+<img src="https://static.igem.org/mediawiki/2011/2/20/Logotec.png" alt="ITESM" name="teclogo" width="146" height="52" id="teclogo">
        <a href="https://2011.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2011/7/73/Logoigemsmall.png" alt="iGEM" name="logoigem" width="70" height="61" id="logoigem"></a>
      <p>&nbsp;</p>
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    		  <div class="panelcontent" style="">
+         <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectoverview">overview</a></p>
-            <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectoverview">overview</a></p>
              <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectparts">parts</a></p>
              <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">genetic frame</a></p>
@@ Line 532: / Line 540: @@
              <p><a href="https://2011.igem.org/Team:Tec-Monterrey/microcongreso">micro congress</a></p>
              <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamfriends">friends</a></p>
   </div>
 		</div>
-       </li><li>
+       </li>
+	  <li>
        <div class="panelcollapsed">
+        <h2>EXTRAS</h2>
-                  <h2>EXTRAS</h2>
    		  <div class="panelcontent">
+			<p><a href="https://2011.igem.org/Team:Tec-Monterrey/sitemap">site map</a></p>
-            <p><a href="https://2011.igem.org/Team:Tec-Monterrey/sitemap">site map</a></p>
              <p><a href="https://2011.igem.org/Team:Tec-Monterrey/sponsorsplatinum">sponsors platinum</a></p>
              <p><a href="https://2011.igem.org/Team:Tec-Monterrey/sponsorsgold">sponsors gold</a></p>
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              <p><a href="https://2011.igem.org/Team:Tec-Monterrey/specialthanks">special thanks</a></p>
              <p><a href="https://2011.igem.org/Team:Tec-Monterrey/followus">follow us!</a></p>
+  		  </div>
-                </div>
+	  </div>
-		</div>
        </li>
-    </ul>
+ </ul>
 </div>
 <div class="content">
-  <!--in sub pages, this divisions should be comments! :D-->
+<div style="
+text-align:center;">
-  <div class="quicklinks">
+ <!--in sub pages, this divisions should be comments! :D-->
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+<br>
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-       </p>
-       <p>&nbsp;</p>
+<center><img src="https://static.igem.org/mediawiki/2011/9/94/Abstractimg01.png" alt="" name="" width="200" height="50" id="tgo"> </center>
+<br>
-      The first DNA construction our team used, was called “Construct C” this assembly was made in order to obtain data from our AraC-Pbad promoter. Our team worked with parts from British Columbia 09 team. Part BBa_K206000, pBAD,  is an E.coli promoter that is induced by L-arabinose. In the absence of arabinose, the repressor protein AraC, BBa_I13458, binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription.
 <br>
-K206000 is a variant pBAD promoter with a modified AraI1 site that has been shown both to be responsive to lower concentrations of arabinose and to exhibit a higher maximum expression than the wild type (BBa_I13453), as measured by coupling to a fluorescent reporter by iGEM09 British Columbia Team.
-We aslo used as a RBS the Kozak sequence used normally on most constructs, part BBa_B0034, followed by a Green Fluorecent Protein (BBa_b0040), supposed to act as a reporter in order to meassure effectivness of the arabinose promoter. This construct was meassured in e. coli’s strain BW27783, which is unable to metabolize arabinose, (our inductor).
 <br>
-Our results meassured by our team member Fausto Ortiz, were the next ones:
-<br>
-Schlief, R. (2000). Regulation of the L-arabinose operon of Escherichia coli. Trends in Genetics. 16(12):559-565.
 </p>
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+<hr>
+<br/>
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+<input type="button" value=" Down " onmouseover="scrollDown()" onmouseout="clearTimeout(t1)">
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-  </div>
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Team:Tec-Monterrey/projectmodeling

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Revision as of 03:47, 29 September 2011

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