Team:UTP-Panama/Experiments

BCCS-Bristol 2010 Biobrick BBa_k381001

First experience with BBa_k381001

Objective:
1. Test operation in liquid LB at 37 ° C and to K2NO3 50 mM.

Methodology:

1. We used six falcon tubes of 25 mL. Three tubes were used for inoculation of the colonies from 06/09/11 and 12/09/11.

2. Two positive controls were prepared and one negative control for each test.

3. To positive control tubes we added 10 ml of liquid LB, 10 mL of chloramphenicol (33μg/mL) and 500 µL of K2NO3 50 mM.

4. Al tubo para control negativo se le agrego lo mismo, excepto K2NO3. To negative control tube we added the same amount specified in the previous line, except K2NO3. 5. Each tube was inoculated with the colonies; three of them with the conlonies from 06/09/11 and the rest with colonies from 12/09/11.

6. Tubes were incubated at 37 ° C for 48 hours.