Team:Baltimore/Project

From 2011.igem.org

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
 
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This is a template page. READ THESE INSTRUCTIONS.
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
 
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:Baltimore_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:Baltimore_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:Baltimore | Team Example]]
 
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Baltimore|Home]]
 
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!align="center"|[[Team:Baltimore/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Baltimore Official Team Profile]
 
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!align="center"|[[Team:Baltimore/Project|Project]]
 
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!align="center"|[[Team:Baltimore/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Baltimore/Modeling|Modeling]]
 
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!align="center"|[[Team:Baltimore/Notebook|Notebook]]
 
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!align="center"|[[Team:Baltimore/Safety|Safety]]
 
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!align="center"|[[Team:Baltimore/Attributions|Attributions]]
 
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== '''Overall project''' ==
 
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Your abstract
 
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TEAM BALTIMORE'S ABSTRACT
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The overall mission of our team is to attempt to overcome some practical barriers to entry of groups and laboratories that may not be well funded or may not have the capital requirements to realize their synthetic biology dreams. Our goal this year is to provide a way for anyone to be able to make their own Taq polymerase instead of having to purchase it. However, the gene for Taq polymerase has a PstI site located within the coding sequence which makes it incompatible with the BioBrick assembly standard. In order to make the Taq polymerase compatible with the BioBrick assembly standard, we need to change the sequence through site directed mutagenesis in a way that would maintain the amino acid sequence of the protein but change the DNA sequence of the gene. In addition, we will be adding the Bio-brick prefix and suffix as well as promoter, terminator and ribosome binding site. Finally, we will be able to generate our own Taq polymerase. We will test the protein to check if our Taq polymerase is active, specific and robust in comparison to the commercial Taq polymerase. If we are successful then this polymerase will reduce the cost of the one of the most expensive reagents in synthetic biology.
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== Project Details==
== Project Details==
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While we designed the point-mutagenesis primers, we took the opportunity to design and order the primers for the BioBrick Suffix and Prefix. We followed the examples laid out in the Registry of Standard Parts for designing the oligos needed to make a part. 
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Important considerations are melting point and CG concentration, as well as self-dimerizations and hairpins. We analyzed these primers using the [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ OligoAnalyzer] from [http://www.idtdna.com/Home/Home.aspx IDT]. When analyzing PolI, only the coding seuence itself was used for sequence inquiry, not the BioBrick Suffix/Prefixes.<br>
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=== Part 2 ===
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 03:09, 29 September 2011


Home Team Project Parts Submitted to the Registry Modeling Notebook Solutions Safety Attributions Hardware Material Safety Data Sheets [http://partsregistry.org/assembly/libraries.cgi?id=35|MIT Parts Registry]


TEAM BALTIMORE'S ABSTRACT

The overall mission of our team is to attempt to overcome some practical barriers to entry of groups and laboratories that may not be well funded or may not have the capital requirements to realize their synthetic biology dreams. Our goal this year is to provide a way for anyone to be able to make their own Taq polymerase instead of having to purchase it. However, the gene for Taq polymerase has a PstI site located within the coding sequence which makes it incompatible with the BioBrick assembly standard. In order to make the Taq polymerase compatible with the BioBrick assembly standard, we need to change the sequence through site directed mutagenesis in a way that would maintain the amino acid sequence of the protein but change the DNA sequence of the gene. In addition, we will be adding the Bio-brick prefix and suffix as well as promoter, terminator and ribosome binding site. Finally, we will be able to generate our own Taq polymerase. We will test the protein to check if our Taq polymerase is active, specific and robust in comparison to the commercial Taq polymerase. If we are successful then this polymerase will reduce the cost of the one of the most expensive reagents in synthetic biology.

Project Details

While we designed the point-mutagenesis primers, we took the opportunity to design and order the primers for the BioBrick Suffix and Prefix. We followed the examples laid out in the Registry of Standard Parts for designing the oligos needed to make a part. Important considerations are melting point and CG concentration, as well as self-dimerizations and hairpins. We analyzed these primers using the [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ OligoAnalyzer] from [http://www.idtdna.com/Home/Home.aspx IDT]. When analyzing PolI, only the coding seuence itself was used for sequence inquiry, not the BioBrick Suffix/Prefixes.

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