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Primer aliquots
From 2011.igem.org
(Difference between revisions)
(Created page with "[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back''']") |
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| + | '''Material:''' | ||
| + | |||
| + | 100mM primer stock solution | ||
| + | |||
| + | Molecular grade H2O | ||
| + | |||
| + | '''Procedure:''' | ||
| + | |||
| + | 1. UV the microtubes to be used for 15 min | ||
| + | |||
| + | 2. thaw out the primer stocks ON ice. | ||
| + | |||
| + | 3. add 10 ul of the stock solution to the microtube | ||
| + | |||
| + | 4. add 90 ul of Molecular grade H2O. | ||
| + | |||
| + | 5. store at -20°C | ||
| + | |||
| + | |||
[https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back'''] | [https://2011.igem.org/Team:Panama/Protocols/Wetlab '''Back'''] | ||
Latest revision as of 03:08, 29 September 2011
Material:
100mM primer stock solution
Molecular grade H2O
Procedure:
1. UV the microtubes to be used for 15 min
2. thaw out the primer stocks ON ice.
3. add 10 ul of the stock solution to the microtube
4. add 90 ul of Molecular grade H2O.
5. store at -20°C
