Team:Lethbridge/Notebook

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<BLOCKQUOTE>
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==Lab Books==
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*<font size=2><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group1 Team 1]:  Amigos Dee, Nathan, Boris, and Ben</b>
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:"Boris said Nathan claimed Ben testified that Dee told everyone to work on Antigen43 and ''Bam''HI or they would no longer be amigos."
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<br>
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*<b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group2 Team 2]:  Comrades Ryan, Dustin, and Terrance</b>
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:"Rise comrades! Rise against the tyranny of stop codons in our scar sequences! Unite all xylene degradation genes and mms6 genes with polypeptide tags!"
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<br>
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*<b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group3 Team 3]:  Spectacular Un-conjoined Non-Twin Anti-Siblings Sutherland and Isaac</b>
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:"Hear the shockingly scientific tale of Lumazine Synthase imaging."
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<br>
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*<b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Justin Justin]:  The Sumerian God of Bicycles</b>
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:"My name is Justin Vigar, undergraduate student of undergraduate students: Look on my lumazine synthase proof of principle for biobrick construction, ye mighty, and despair."
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<br>
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==<font color="black">Protocols==
==<font color="black">Protocols==
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<font size=2><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Common_Protocols Common Protocols]</b></font>
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<font size=4><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Freezer_Stocks Student Freezer Stocks]</b></font>
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==Freezer Stocks==
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==Group Pages==
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<font size=2><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Freezer_Stocks Student Freezer Stocks]</b></font>
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*<font size=3><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group1 Group 1]<br>
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<br><br>
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*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group2 Group 2]<br>
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*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group3 Group 3]<br>
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==Assignments==
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===April 9th - April 30th, 2011===
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====Group 1 (JV, DG, AV, BA, DM, IW)====
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#<u>Assembly Round 1</u>
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##K331033 + K331035
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##R0040 + K331023 ==> K331031 (Already Made)
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##K331025 + B0015
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##R0040 + K331039
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##K331039 + B0015
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#<u>Assembly Round 2</u>
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## 1.2 + 1.3
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## 1.4 + B0015
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## R0040 + 1.4
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Characterize Fluorescence of Parts 1.1, 2.1, 2.2, 2.3
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====Group 2 (HB, BL, MC, BV)====
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#<u>Assembly Round 1</u>
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##R0010 + B0034
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##K249002 + B0015
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##I13452 + B0034
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##C0040 + B0015
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#<u>Assembly Round 2</u>
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## 1.1 + 1.2
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## 1.3 + 1.4
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#<u>Assembly Round 3</u>
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## 2.1 + 2.2
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====Group 3 (JS, RP, KG, CC, SD)====
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*Use PCR to alter suffixes to include fusion standards for xylE and mms6
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*Assemble fusion-ready xylE and mms6 with S04261 (C-terminal Arginine tail + B0015)
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*Move these BioBricks from synthesis vectors (either pMA-T or pMA-RQ) into pSB1C3
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**K331007
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**K331008
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**K331022
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**K331024
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**K331025
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Latest revision as of 02:50, 29 September 2011




Lab Books

  • Team 1: Amigos Dee, Nathan, Boris, and Ben
"Boris said Nathan claimed Ben testified that Dee told everyone to work on Antigen43 and BamHI or they would no longer be amigos."


  • Team 2: Comrades Ryan, Dustin, and Terrance
"Rise comrades! Rise against the tyranny of stop codons in our scar sequences! Unite all xylene degradation genes and mms6 genes with polypeptide tags!"


  • Team 3: Spectacular Un-conjoined Non-Twin Anti-Siblings Sutherland and Isaac
"Hear the shockingly scientific tale of Lumazine Synthase imaging."


  • Justin: The Sumerian God of Bicycles
"My name is Justin Vigar, undergraduate student of undergraduate students: Look on my lumazine synthase proof of principle for biobrick construction, ye mighty, and despair."


Protocols

Common Protocols

Freezer Stocks

Student Freezer Stocks

Retrieved from "http://2011.igem.org/Team:Lethbridge/Notebook"