Team:Lethbridge/Notebook

From 2011.igem.org

(Difference between revisions)
(→Group 3 (JS, RP, KG, CC, SD))
(→Lab Books)
 
(35 intermediate revisions not shown)
Line 1: Line 1:
-
<div style="background-color:#000000; color:white">
+
{{TeamLethbridgeHead}}
 +
 
 +
<div style="background-color:#FFFFFF; color:black">
<html>
<html>
<br>
<br>
-
<table border="0" width="100%" style="background-color:#000000">
+
<table border="0" width="100%" style="background-color:#FFFFFF">
<tr>
<tr>
Line 10: Line 12:
<th>
<th>
-
<image src="https://static.igem.org/mediawiki/2011/9/95/Lethbridgelogo2011.jpg" width="200px"/>
+
<image src="https://static.igem.org/mediawiki/2011/9/93/Notebook.jpg" width="200px"/>
</th>
</th>
Line 16: Line 18:
<th>
<th>
-
<image src="https://static.igem.org/mediawiki/2011/d/d5/Lethbridge2010teamphoto.jpg" height="300px"/>
+
<image src="https://static.igem.org/mediawiki/2011/3/31/Picture25.jpg" height="340px" width="500px"/>
</th>
</th>
Line 22: Line 24:
<th>
<th>
-
<image src="https://static.igem.org/mediawiki/2011/9/95/Lethbridgelogo2011.jpg" width="200px"/>
 
</th>
</th>
Line 30: Line 31:
</table>
</table>
-
 
-
 
-
<br>
 
-
 
-
<align="centre">
 
-
<table border="0"  width="100%"  style="background-color:#000000">
 
-
 
-
<tr>
 
-
 
-
<th>
 
-
 
-
<a href="https://2011.igem.org/Team:Lethbridge">
 
-
<img src="https://static.igem.org/mediawiki/2010/2/22/UofLHome.jpg" width="80"/>
 
-
</a>
 
-
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Team">
 
-
<img src="https://static.igem.org/mediawiki/2010/0/0d/UofLTeam.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Project">
 
-
<img src="https://static.igem.org/mediawiki/2010/8/8d/UofLProjectbutton.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Notebook">
 
-
<img src="https://static.igem.org/mediawiki/2010/7/73/UofLNotebookbutton.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Parts">
 
-
<img src="https://static.igem.org/mediawiki/2010/8/84/UofLPartsSubmittedToTheRegistrybutton.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Modeling">
 
-
<img src="https://static.igem.org/mediawiki/2010/e/e1/UofLModelingbutton.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Ethics">
 
-
<img src="https://static.igem.org/mediawiki/2010/2/26/UofLEthicsbutton.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Safety">
 
-
<img src="https://static.igem.org/mediawiki/2010/0/00/UofLSafetybutton.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Art">
 
-
<img src="https://static.igem.org/mediawiki/2010/0/0a/UofLArt.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/News">
 
-
<img src="https://static.igem.org/mediawiki/2010/c/c3/UofLNewsButton.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
 
-
<th><a href="https://2011.igem.org/Team:Lethbridge/Attributions">
 
-
<img src="https://static.igem.org/mediawiki/2011/2/2e/UofLattributionsbutton.jpg" width="80"/>
 
-
</a>
 
-
</th>
 
-
</table>
 
</body>
</body>
</html>
</html>
 +
<br>
<hr>
<hr>
 +
<br>
 +
<BLOCKQUOTE>
 +
==Lab Books==
 +
*<font size=2><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group1 Team 1]:  Amigos Dee, Nathan, Boris, and Ben</b>
 +
:"Boris said Nathan claimed Ben testified that Dee told everyone to work on Antigen43 and ''Bam''HI or they would no longer be amigos."
 +
<br>
 +
*<b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group2 Team 2]:  Comrades Ryan, Dustin, and Terrance</b>
 +
:"Rise comrades! Rise against the tyranny of stop codons in our scar sequences! Unite all xylene degradation genes and mms6 genes with polypeptide tags!"
 +
<br>
 +
*<b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group3 Team 3]:  Spectacular Un-conjoined Non-Twin Anti-Siblings Sutherland and Isaac</b>
 +
:"Hear the shockingly scientific tale of Lumazine Synthase imaging."
 +
<br>
 +
*<b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Justin Justin]:  The Sumerian God of Bicycles</b>
 +
:"My name is Justin Vigar, undergraduate student of undergraduate students: Look on my lumazine synthase proof of principle for biobrick construction, ye mighty, and despair."
 +
<br>
 +
</font>
-
==<font color="white">Group Member Pages==
+
==<font color="black">Protocols==
-
*<font size=3><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group1 JV & DG]<br>
+
<font size=2><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Common_Protocols Common Protocols]</b></font>
-
*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group2 AV & IW]<br>
+
-
*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group3 JS & RP]<br>
+
-
*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group4 HB & BL]<br>
+
-
*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group5 MC & BV]<br>
+
-
*[https://2011.igem.org/Team:Lethbridge/Notebook/Lab_Work/Group6 KG & SD]<br></b></font>
+
-
==Assignments==
+
==Freezer Stocks==
-
===April 9th - April 30th, 2011===
+
<font size=2><b>[https://2011.igem.org/Team:Lethbridge/Notebook/Freezer_Stocks Student Freezer Stocks]</b></font>
-
====Group 1 (JV, DG, AV, BA, IW)====
+
-
#<u>Assembly Round 1</u>
+
-
##K331033 + K331035
+
-
##R0040 + K331023 ==> K331031 (Already Made)
+
-
##K331025 + B0015
+
-
##R0040 + K331039
+
-
##K331039 + B0015
+
-
#<u>Assembly Round 2</u>
+
-
## 1.2 + 1.3
+
-
## 1.4 + B0015
+
-
## R0040 + 1.4
+
-
 
+
-
Characterize Fluorescence of Parts 1.1, 2.1, 2.2, 2.3
+
-
 
+
-
====Group 2 (HB, BL, MC, BV)====
+
-
#<u>Assembly Round 1</u>
+
-
##R0010 + B0034
+
-
##K249002 + B0015
+
-
##I13452 + B0034
+
-
##C0040 + B0015
+
-
#<u>Assembly Round 2</u>
+
-
## 1.1 + 1.2
+
-
## 1.3 + 1.4
+
-
#<u>Assembly Round 3</u>
+
-
## 2.1 + 2.2
+
-
 
+
-
====Group 3 (JS, RP, KG, CC, SD)====
+
-
*Use PCR to alter suffixes to include fusion standards for xylE and mms6
+
-
*Assemble fusion-ready xylE and mms6 with S04261 (C-terminal Arginine tail + B0015)
+
-
*Move these BioBricks from synthesis vectors (either pMA-T or pMA-RQ) into pSB1C3
+
-
**K331007
+
-
**K331008
+
-
**K331022
+
-
**K331024
+
-
**K331025
+
-
 
+
-
==Protocols==
+
-
<br>
+
-
[https://2011.igem.org/Team:Lethbridge/Notebook/Freezer_Stocks Student Freezer Stocks]
+
<br><br>
<br><br>

Latest revision as of 02:50, 29 September 2011




Lab Books

  • Team 1: Amigos Dee, Nathan, Boris, and Ben
"Boris said Nathan claimed Ben testified that Dee told everyone to work on Antigen43 and BamHI or they would no longer be amigos."


  • Team 2: Comrades Ryan, Dustin, and Terrance
"Rise comrades! Rise against the tyranny of stop codons in our scar sequences! Unite all xylene degradation genes and mms6 genes with polypeptide tags!"


  • Team 3: Spectacular Un-conjoined Non-Twin Anti-Siblings Sutherland and Isaac
"Hear the shockingly scientific tale of Lumazine Synthase imaging."


  • Justin: The Sumerian God of Bicycles
"My name is Justin Vigar, undergraduate student of undergraduate students: Look on my lumazine synthase proof of principle for biobrick construction, ye mighty, and despair."


Protocols

Common Protocols

Freezer Stocks

Student Freezer Stocks

Retrieved from "http://2011.igem.org/Team:Lethbridge/Notebook"