Team:Calgary/Project/Acomplishments
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- | <b>The University of Calgary iGEM Team has had many accomplishments this summer:</b><br><br> | + | <p><b>The University of Calgary iGEM Team has had many accomplishments this summer:</b></p><br><br> |
- | <b>In our promoter project...</b>< | + | <img style="float:right;" src="https://static.igem.org/mediawiki/2011/b/ba/UCalgary2011_BacterialPromoterSmall.png"></img> |
- | <ul><li>We designed a novel protocol to identify small hydrophobic molecule interacters within a cell.</li> | + | <p><b>In our promoter project...</b></p> |
- | <li>We were able to successfully biotinylate a naphthenic acid, suggesting that we may be able to biotinylate other small hydrophobic molecules to screen for interactors in the search for responsive elements.</li> | + | <ul><li><p>We designed a novel protocol to identify small hydrophobic molecule interacters within a cell.</p></li> |
- | <li>We identified a gene that is upregulated in the presence of naphthenic acids, suggesting a possible responsive elements or degradative protein.</li><br></ul> | + | <li><p>We were able to successfully biotinylate a naphthenic acid, suggesting that we may be able to biotinylate other small hydrophobic molecules to screen for interactors in the search for responsive elements.</p></li> |
- | <b>In our Reporter Project...</b>< | + | <li><p>We identified a gene that is upregulated in the presence of naphthenic acids, suggesting a possible responsive elements or degradative protein.</p></li><br></ul> |
- | <li>We characterized a novel reporter function for the <i>lacZ</i> gene using an electrochemical output.</li> | + | |
- | <li>We laid the groundwork for future use of electrochemical reporting in iGEM.</li><br></ul> | + | <img style="float:right;" src="https://static.igem.org/mediawiki/2011/6/6c/UCalgary2011_BacterialGeneSmall.png"></img> |
- | <b>In our Chassis project...</b>< | + | <p><b>In our Reporter Project...</b></p><ul> |
- | <li>We characterized a<i> Pseudomonas-E. coli</i> conjugation construct</li> | + | <li><p>We characterized a novel reporter function for the <i>lacZ</i> gene using an electrochemical output.</p></li> |
- | <li>We submitted parts and protocols for future work in both microalgae and <i>Pseudomonas</i></li> | + | <li><p>We laid the groundwork for future use of electrochemical reporting in iGEM.</p></li><br></ul> |
- | <li>We characterized the viability of <i>Pseudomonas</i> and <i>E. coli</i> to grow and survive in tailings ponds</li> | + | |
+ | <img style="float:right;" src="https://static.igem.org/mediawiki/2011/0/02/UCalgary2011_BacterialChassisSmall.png"></img> | ||
+ | <p><b>In our Chassis project...</b></p><ul> | ||
+ | <li><p>We characterized a<i> Pseudomonas-E. coli</i> conjugation construct</p></li> | ||
+ | <li><p>We submitted parts and protocols for future work in both microalgae and <i>Pseudomonas</i></p></li> | ||
+ | <li><p>We characterized the viability of <i>Pseudomonas</i> and <i>E. coli</i> to grow and survive in tailings ponds</p></li> | ||
</ul><br> | </ul><br> | ||
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<li><b>Submit at least one new well-characterized standard BioBrick Part</b> - Our team has submitted several new parts all of which we have fully characterized. | <li><b>Submit at least one new well-characterized standard BioBrick Part</b> - Our team has submitted several new parts all of which we have fully characterized. | ||
</ul> | </ul> | ||
- | + | <br> | |
<b>Silver:</b> | <b>Silver:</b> | ||
<ul><li><b>Demonstrate at least one new BioBrick part designed works as expected</b> - All of our parts have been carefully tested. Our oriT-ori1600 <i>Psuedomonas</i> conjugation part has been well characterized.</li> | <ul><li><b>Demonstrate at least one new BioBrick part designed works as expected</b> - All of our parts have been carefully tested. Our oriT-ori1600 <i>Psuedomonas</i> conjugation part has been well characterized.</li> | ||
<li><b>Enter this information and other documentation on the part's 'Main Page' section of the Registry</b> - The data relating to our ne BioBrick parts have all been added to the Registry.</li> | <li><b>Enter this information and other documentation on the part's 'Main Page' section of the Registry</b> - The data relating to our ne BioBrick parts have all been added to the Registry.</li> | ||
</ul> | </ul> | ||
- | + | <br> | |
<b>Gold:</b> | <b>Gold:</b> | ||
- | <ul><li><b>Improve the function of an existing BioBrick Part</b> - Our team utilized the <i>lacZ<i> gene to develop and characterize it as an electrochemical detector. Please see the <a href="https://2011.igem.org/Team:Calgary/Project/Reporter"> reporter section</a> for more information. | + | <ul><li><b>Improve the function of an existing BioBrick Part</b> - Our team utilized the <i>lacZ</i> gene to develop and characterize it as an electrochemical detector. Please see the <a href="https://2011.igem.org/Team:Calgary/Project/Reporter"> reporter section</a> for more information.</li> |
- | <li><b>Help another iGEM Team</b> - The University of Calgary iGEM Team collaberated with the University of Lethridge iGEM Team since both of our projects were involving tailings ponds water. We characterized <i>E. coli</i> viability in our unique tailing ponds samples and developed a well standardized data set by working together. For more information see our <a href="https://2011.igem.org/Team:Calgary/Outreach/Collaboration"> click here</a> | + | <li><b>Help another iGEM Team</b> - The University of Calgary iGEM Team collaberated with the University of Lethridge iGEM Team since both of our projects were involving tailings ponds water. We characterized <i>E. coli</i> viability in our unique tailing ponds samples and developed a well standardized data set by working together. For more information see our <a href="https://2011.igem.org/Team:Calgary/Outreach/Collaboration"> click here</a></li> |
- | <li><b>Outline a new approach to an issue of Human Practice in Synthetic Biology</b> - In Canada, the government has mandated that oilsands companies must test for the presence of naphthenic acids four times a year in their tailings ponds. Obviously public opinion and government policy will influence the actions oil companies will make in Alberta. Our human practices is a report on the role of government and industry on tailings ponds bioremediation and how synthetic biology and our device will fit into the present policies and procedures implemented in Alberta. Please <a href="https://2011.igem.org/Team:Calgary/Outreach/Reportn"> click here</a> for more information. | + | <li><b>Outline a new approach to an issue of Human Practice in Synthetic Biology</b> - In Canada, the government has mandated that oilsands companies must test for the presence of naphthenic acids four times a year in their tailings ponds. Obviously public opinion and government policy will influence the actions oil companies will make in Alberta. Our human practices is a report on the role of government and industry on tailings ponds bioremediation and how synthetic biology and our device will fit into the present policies and procedures implemented in Alberta. Please <a href="https://2011.igem.org/Team:Calgary/Outreach/Reportn"> click here</a> for more information.</li> |
- | + | </ul> | |
</html> | </html> | ||
}} | }} |
Revision as of 02:34, 29 September 2011
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Accomplishments
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The University of Calgary iGEM Team has had many accomplishments this summer:
![](https://static.igem.org/mediawiki/2011/b/ba/UCalgary2011_BacterialPromoterSmall.png)
In our promoter project...
We designed a novel protocol to identify small hydrophobic molecule interacters within a cell.
We were able to successfully biotinylate a naphthenic acid, suggesting that we may be able to biotinylate other small hydrophobic molecules to screen for interactors in the search for responsive elements.
We identified a gene that is upregulated in the presence of naphthenic acids, suggesting a possible responsive elements or degradative protein.
![](https://static.igem.org/mediawiki/2011/6/6c/UCalgary2011_BacterialGeneSmall.png)
In our Reporter Project...
We characterized a novel reporter function for the lacZ gene using an electrochemical output.
We laid the groundwork for future use of electrochemical reporting in iGEM.
![](https://static.igem.org/mediawiki/2011/0/02/UCalgary2011_BacterialChassisSmall.png)
In our Chassis project...
We characterized a Pseudomonas-E. coli conjugation construct
We submitted parts and protocols for future work in both microalgae and Pseudomonas
We characterized the viability of Pseudomonas and E. coli to grow and survive in tailings ponds
Judging Requirements
Bronze:- Team registration - Our team successfully registered for the iGEM Competition.
- Complete Judging Form - To view our Judging Form please click here.
- Team Wiki - Our team has successfully designed a wiki page for our project.
- Submit at least one new well-characterized standard BioBrick Part - Our team has submitted several new parts all of which we have fully characterized.
Silver:
- Demonstrate at least one new BioBrick part designed works as expected - All of our parts have been carefully tested. Our oriT-ori1600 Psuedomonas conjugation part has been well characterized.
- Enter this information and other documentation on the part's 'Main Page' section of the Registry - The data relating to our ne BioBrick parts have all been added to the Registry.
Gold:
- Improve the function of an existing BioBrick Part - Our team utilized the lacZ gene to develop and characterize it as an electrochemical detector. Please see the reporter section for more information.
- Help another iGEM Team - The University of Calgary iGEM Team collaberated with the University of Lethridge iGEM Team since both of our projects were involving tailings ponds water. We characterized E. coli viability in our unique tailing ponds samples and developed a well standardized data set by working together. For more information see our click here
- Outline a new approach to an issue of Human Practice in Synthetic Biology - In Canada, the government has mandated that oilsands companies must test for the presence of naphthenic acids four times a year in their tailings ponds. Obviously public opinion and government policy will influence the actions oil companies will make in Alberta. Our human practices is a report on the role of government and industry on tailings ponds bioremediation and how synthetic biology and our device will fit into the present policies and procedures implemented in Alberta. Please click here for more information.
![](https://static.igem.org/mediawiki/2011/c/cb/UCalgary_BodyBoxBottom.png)