Team:ITESM Mexico/Assembly
From 2011.igem.org
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Usage and Biology: This promoter contains upstream regions of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC. | Usage and Biology: This promoter contains upstream regions of the ompC porin gene. The regulation of ompC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC. | ||
Activation of ompC has been experimentally shown to occur with just the C1 OmpR binding region, provided that the C1 region is placed next to the -35 signal (Maeda and Mizuno, 1990). This promoter is a truncated version of BBa_R0082, which contains C1, C2, and C3. | Activation of ompC has been experimentally shown to occur with just the C1 OmpR binding region, provided that the C1 region is placed next to the -35 signal (Maeda and Mizuno, 1990). This promoter is a truncated version of BBa_R0082, which contains C1, C2, and C3. | ||
+ | Ampicillin resistant. | ||
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+ | [[File:RecA.jpg|100px|caption]] | ||
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+ | '''BBa_K587001:''' RecA protein coding sequence | ||
+ | Ampicillin resistant. | ||
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+ | [[File:LambdaRepresor.jpg|100px|caption]] | ||
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+ | '''BBa_C0051:''' cI repressor from E. coli phage lambda (+LVA) | ||
+ | Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051). | ||
+ | Ampicillin resistant. | ||
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+ | [[File:Or3.jpg|100px|caption]] | ||
+ | <html></td><td></html> | ||
+ | '''BBa_K079043:''' Lambda OR3 - operator library member | ||
+ | Lambda Genetic switch is a complex and deeply characterized system. | ||
+ | Ampicillin resistant. | ||
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+ | |||
+ | <html><tr><td></html> | ||
+ | [[File:Or2.jpg|100px|caption]] | ||
+ | <html></td><td></html> | ||
+ | '''BBa_K079042:''' Lambda OR2 - operator library member. Lambda Genetic switch is a complex and deeply characterized system. | ||
+ | Ampicillin resistant. | ||
+ | <html></td></tr></html> | ||
+ | |||
+ | <html><tr><td></html> | ||
+ | [[File:Or1.jpg|100px|caption]] | ||
+ | <html></td><td></html> | ||
+ | '''BBa_K079041:''' Lambda OR1 - operator library member. Lambda Genetic switch is a complex and deeply characterized system. | ||
Ampicillin resistant. | Ampicillin resistant. | ||
<html></td></tr></html> | <html></td></tr></html> |
Revision as of 01:57, 29 September 2011