Team:UC Davis/Data LambdacI

From 2011.igem.org

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<h1 id="widget"> λ cI Promoter Characterization Data</h1>
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<h1>λ c1 Promoter Data</h1>
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<div id="mutantwidget" class="floatbox2">
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<h3>Wildtype</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352</span>
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<span class="stdevs"> 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456</span>
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<span class="numconditions">4</span>
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<h3>Mutant 1</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.6492, 0.20526, 0.17329, 0.16899, 0.16682, 0.16631, 0.16913</span>
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<span class="stdevs"> 0.017682, 0.0032754, 0.0010138, 0.0011266, 0.00097033, 0.0012072, 0.0011179</span>
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<h3>Mutant 2</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.53303, 0.16064, 0.14171, 0.12149, 0.12547, 0.11146, 0.1061</span>
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<span class="stdevs"> 0.02651, 0.0029121, 0.0027198, 0.0033112, 0.0034421, 0.0059837, 0.0027095</span>
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<h3>Mutant 4</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.23, 0.038825, 0.035663, 0.036119, 0.035423, 0.034289, 0.032858</span>
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<span class="stdevs"> 0.02048, 0.00047265, 0.00028731, 0.00037973, 0.00031652, 0.00026622, 0.00070058</span>
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<h3>Mutant 5</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.92311, 0.23346, 0.1833, 0.17092, 0.16534, 0.16473, 0.15538</span>
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<span class="stdevs"> 0.0061434, 0.0029621, 0.0016015, 0.0037633, 0.0029015, 0.002096, 0.0024785</span>
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<h3>Mutant 6</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.97182, 0.98592, 1.01000, 1.02300, 1.03610, 1.04490, 1.01750</span>
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<span class="stdevs"> 0.018621, 0.01966, 0.018634, 0.018826, 0.018993, 0.020762, 0.019842</span>
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<h3>Mutant 7</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.48325, 0.13919, 0.12353, 0.10617, 0.10943, 0.099213, 0.08565</span>
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<span class="stdevs"> 0.012073, 0.0025601, 0.0030506, 0.0027667, 0.002179, 0.0022998, 0.0015159</span>
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<h3>Mutant 8</h3>
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<span class="sequence">SEQUENCE GOES HERE!</span>
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<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
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<span class="ydata"> 0.41657, 0.12325, 0.10744, 0.10365, 0.10006, 0.096006, 0.09219</span>
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<span class="stdevs"> 0.0080566, 0.0028055, 0.0027614, 0.0038199, 0.0038188, 0.0024541, 0.0022523</span>
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The above widget displays the relative GFP fluorescence data that we have collected for the eight lambda cI mutant promoters and the wild type R0051 promoter. The plots are normalized to the wild type such that the wild type's fluorescence at 0% arabinose and 0 mM IPTG is 1. The data for these plots were gathered using the constructs outlined on the <a href="https://2011.igem.org/Team:UC_Davis/cilambda">lambda cI project page</a>, which were designed so that increasing the arabinose concentration would proportionally increase the repressor concentration. This widget allows you to toggle the display of whichever mutants you would like to view and easily observe the changes that these mutations cause.
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We are still in the process of screening λ c1 promoters for GFP activity and do not have final data to to present at this time.<br><br>
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Check back here for updates after the American regional comepetition!
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<h1>Analysis</h1>
 
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It is evident from the above plots that the variants in this lambda cI mutant library vary quite a bit in maximal promoter activity and in repressor sensitivity, especially when compared to wild type. In this data set, we see a very good range in activity level when there is minimal repressor present, but when the concentration of repressor is increased, the promoters quickly become fully repressed. This could mean that we have increased the binding affinity of these sequences to the lambda cI repressor, but we would need to examine sequences before we can know for sure. Additionally, with Mutant #6, it appears as though we have eliminated the ability of the repressor to bind all together. Once again, sequences for this part would be necessary to confirm this suspicion. Nonetheless, having these various levels of basal promoter activity is very useful and has many potential <a href="https://2011.igem.org/Team:UC_Davis/Project_Selection">applications to synthetic biology</a>.
 
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Latest revision as of 01:50, 29 September 2011

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Criteria

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λ c1 Promoter Data

We are still in the process of screening λ c1 promoters for GFP activity and do not have final data to to present at this time.

Check back here for updates after the American regional comepetition!

Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LambdacI"