Team:UT Dallas/notebook dates
From 2011.igem.org
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Latest revision as of 01:40, 29 September 2011
Calendar
Week 1
7/11/2011 | Preparing sent parts |
7/12/2011 |
Preparing sent parts growing LB and appropriate antibiotic overnight |
7/13/2011 |
all grew, negative controls didn't Preparing sent parts, take 200µL from LB grown overnight and plated on appropriate antibiotic |
7/14/2011 |
all grew a blob and a few colonies along the edge Transformation at 37 degrees Celsius overnight- 50 µL DH5a, 2 µL DNA (all carb) Plating sent parts Take one colony from July 13 plate properly |
7/15/2011 |
Transformation of 50µL of DH5a, 2 µL DNA (all carb) at 37 degrees overnight Incubated in 3mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm. |