Team:Nevada/iGEMcollaborators

From 2011.igem.org

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<b><u>Contribution to the pSB1C3</u>
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<b><u>Contribution to the pSB1C3</u><p>
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The iGEM team Nevada contributed with correction of pSB1C3. Megan Tabor detected the error in the pSB1C3(the chloramphenicol resistant backbone).  pSB1C3 is the submission plasmid for iGEM and we were also using it for our our thiamnie knockout construct that will make the Synacasistis an auxotrouph.  When trying to make the primers for isolation of the chloramphenical resistance protein from the plasmid using PCR, the mapped out section did not blast as chlormamphenical resistance and did not fit with the ORF. Megan contacted the MIT team that created the pSB1C3 to find more information and a correction. Below are the emails of the conversation.
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The iGEM team Nevada contributed with correction of pSB1C3. Megan Tabor detected the error in the pSB1C3(the chloramphenicol resistant backbone).  pSB1C3 is the submission plasmid for iGEM and we were also using it for our our thiamnie knockout construct that will make the Synacasistis an auxotrouph.  When trying to make the primers for isolation of the chloramphenical resistance protein from the plasmid using PCR, the mapped out section did not blast as chlormamphenical resistance and did not fit with the ORF. Megan contacted the MIT team that created the pSB1C3 to find more information and a correction. Below are the emails of the conversation.<p>
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From: Megan Tabor <tabormi22@gmail.com>
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From: Megan Tabor <tabormi22@gmail.com><p>
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Date: Mon, Aug 1, 2011 at 4:53 PM
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Date: Mon, Aug 1, 2011 at 4:53 PM<p>
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Subject: iGEM Team Nevada 2011
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Subject: iGEM Team Nevada 2011<p>
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To: webmail@austinche.name
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To: webmail@austinche.name<p>
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Hello Austin,
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Hello Austin,<p>
My name is Megan Tabor and I am a currant iGEM member.  I am trying to work with the plasmid backbone pSB1C3 ( the chloramphenicol resistant backbone).  I would like to isolate it out of the plasmid but there does not seem to be a start or stop codon associated with where on the plasmid the resistance is supposed to be.  I was wondering if you know for sure that is resistance is where it was stated.  Thank you so much for your time.<p>
My name is Megan Tabor and I am a currant iGEM member.  I am trying to work with the plasmid backbone pSB1C3 ( the chloramphenicol resistant backbone).  I would like to isolate it out of the plasmid but there does not seem to be a start or stop codon associated with where on the plasmid the resistance is supposed to be.  I was wondering if you know for sure that is resistance is where it was stated.  Thank you so much for your time.<p>
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Megan Tabor
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Megan Tabor<p>
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---------- Forwarded message -------------------
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---------- Forwarded message -------------------<p>
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From: Austin Che <austin@csail.mit.edu>
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From: Austin Che <austin@csail.mit.edu><p>
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Date: Tue, Aug 2, 2011 at 6:06 AM
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Date: Tue, Aug 2, 2011 at 6:06 AM<P>
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Subject: Re: iGEM Team Nevada 2011
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Subject: Re: iGEM Team Nevada 2011<P>
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To: Megan Tabor <tabormi22@gmail.com>
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To: Megan Tabor <tabormi22@gmail.com><p>
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 The annotation on the registry was off by 2 bases on either side. I've updated the registry info.
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 The annotation on the registry was off by 2 bases on either side. I've updated the registry info.<p>

Revision as of 23:51, 28 September 2011



Contribution to the pSB1C3

The iGEM team Nevada contributed with correction of pSB1C3. Megan Tabor detected the error in the pSB1C3(the chloramphenicol resistant backbone). pSB1C3 is the submission plasmid for iGEM and we were also using it for our our thiamnie knockout construct that will make the Synacasistis an auxotrouph. When trying to make the primers for isolation of the chloramphenical resistance protein from the plasmid using PCR, the mapped out section did not blast as chlormamphenical resistance and did not fit with the ORF. Megan contacted the MIT team that created the pSB1C3 to find more information and a correction. Below are the emails of the conversation.

From: Megan Tabor

Date: Mon, Aug 1, 2011 at 4:53 PM

Subject: iGEM Team Nevada 2011

To: webmail@austinche.name

Hello Austin,

My name is Megan Tabor and I am a currant iGEM member.  I am trying to work with the plasmid backbone pSB1C3 ( the chloramphenicol resistant backbone).  I would like to isolate it out of the plasmid but there does not seem to be a start or stop codon associated with where on the plasmid the resistance is supposed to be.  I was wondering if you know for sure that is resistance is where it was stated.  Thank you so much for your time.

Megan Tabor

---------- Forwarded message -------------------

From: Austin Che

Date: Tue, Aug 2, 2011 at 6:06 AM

Subject: Re: iGEM Team Nevada 2011

To: Megan Tabor

 The annotation on the registry was off by 2 bases on either side. I've updated the registry info.


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