Team:Panama/BioBricks

From 2011.igem.org

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(Re-designing and re-assembling of the rhamnosyltransferase BioBrick part:"The Biosurfactator")
 
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== '''Re-designing and re-assembling of the rhamnosyltransferase BioBrick part:''' '''"The Biosurfactator"''' ==
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{{:Team:Panama/template|title=BioBricks}}
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== '''Re-designing and re-assembling of the rhamnosyltransferase BioBrick part:"The Biosurfactator"''' ==
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The design and construction of the rhamnosyltransferase gene into a BioBrick part was the main goal and achievement of the iGEM Panama Team 2010, but since the beginning of this year’s iGEM project we failed in producing the rhamnolipid compound into our ''E. coli'' based-factory, because our last year’s BioBrick appeared to have been denaturalized. We then ordered our BioBrick part BBa_K424018 (iGEM Panama team 2010) from The Registry and once it arrived we ran some tests and we noticed that the part was no longer fitted into the plasmid backbone.
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The design and construction of the rhamnosyltransferase gene into a BioBrick part was the main goal and achievement of the iGEM Panama Team 2010, but since the beginning of this year’s iGEM project we failed in producing the rhamnolipid compound into our ''E. coli'' based-factory, because our last year’s BioBrick appeared to have been denaturalized. We then ordered our BioBrick part [http://partsregistry.org/wiki/index.php/Part:BBa_K424018 BBa_K424018] (iGEM Panama team 2010) from The Registry and once it arrived we ran some tests and we noticed that the part was no longer fitted into the plasmid backbone.
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We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the gene that is responsible to synthesize the key enzyme rhamnosyltranferase (RhlAB) to be compatible with Assembly Standard 10. We have achieved this by performing several silent mutations using the QuikLighting Multi Site-Directed Mutagenesis Kit from Stratagene. This rhamnosyltransferase BioBrick (Rh1AB_BB) is ready to be tested on an expression plasmid device following the Assembly Standard Protocol 10.
We developed this standarized part to be inserted into E. coli for rhamnolipid production through the mutation of the gene that is responsible to synthesize the key enzyme rhamnosyltranferase (RhlAB) to be compatible with Assembly Standard 10. We have achieved this by performing several silent mutations using the QuikLighting Multi Site-Directed Mutagenesis Kit from Stratagene. This rhamnosyltransferase BioBrick (Rh1AB_BB) is ready to be tested on an expression plasmid device following the Assembly Standard Protocol 10.
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[http://partsregistry.org/Part:BBa_K653000:Design '''See our 2011 BioBrick part here!''']

Latest revision as of 23:01, 28 September 2011