Team:Michigan/Parts

From 2011.igem.org

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{{Michigan_Header2}}
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==Abstract==
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The ability of zinc finger domains to selectively bind specific double stranded DNA sequences have largely been applied intracellularly, such as in engineered zinc finger nucleases for genomic manipulations. Proteins containing zinc finger domains can also be used extracellularly to precisely adhere objects to surfaces containing bound oligonucleotides. This project aims to utilize the specificity of zinc finger protein to direct binding of Escherichia coli to oligonucleotides bound on surfaces. The fusion protein engineered to contain a fragment of the OmpA membrane domain and a zinc finger domain allows the protein to be expressed on the outside of the cell while remaining bound to the host cell.
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==Data Page==
==Data Page==
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'''Data For Our Favorite New Parts'''
'''Data For Our Favorite New Parts'''
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1. LacI regulated promoter-OmpA-linker-Zif268 fusion protein, [[BBa_XXXXXX (main page)]]: This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Zif268-HIV DNA binding domain, BBa_K165006 ([http://partsregistry.org/Part:BBa_K165006 main page]), controlled by the LacI regulated promoter, BBa_J04500 ([http://partsregistry.org/Part:BBa_J04500 main page]).
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1. OmpA-linker-Zif268 fusion protein controlled by LacI regulated promoter, [http://partsregistry.org/Part:BBa_K618212 (main page)]: This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Zif268-HIV DNA binding domain, BBa_K165006 ([http://partsregistry.org/Part:BBa_K165006 main page]), controlled by the LacI regulated promoter, BBa_J04500 ([http://partsregistry.org/Part:BBa_J04500 main page]).
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2. LacI regulated promoter-OmpA-linker-Gli1 fusion protein, [[BBa_XXXXXX (main page)]]:
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2. OmpA-linker-Gli1 fusion protein controlled by LacI regulated promoter, [http://partsregistry.org/Part:BBa_K618211 (main page)]:
This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Gli-1 DNA binding domain, BBa_K165007 ([http://partsregistry.org/Part:BBa_K165007 main page]), controlled by the LacI regulated promoter, BBa_J04500 ([http://partsregistry.org/Part:BBa_J04500 main page]).
This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Gli-1 DNA binding domain, BBa_K165007 ([http://partsregistry.org/Part:BBa_K165007 main page]), controlled by the LacI regulated promoter, BBa_J04500 ([http://partsregistry.org/Part:BBa_J04500 main page]).
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3. AraB promoter-OmpA-linker-Zif268 fusion protein, [[BBa_XXXXXX (Main page)]]:
 
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4. AraB promoter-OmpA-linker-Gli1 fusion protein, [[BBa_XXXXXX (Main page)]]:
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3. OmpA-linker-Zif268 fusion protein controlled by arabinose regulated promoter (AraC/pBAD), [http://partsregistry.org/Part:BBa_K618112 (main page)]: This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Zif268-HIV DNA binding domain, BBa_K165006 ([http://partsregistry.org/Part:BBa_K165006 main page]), controlled by the arabinose-regulated promoter, Bba_K259007 ([http://partsregistry.org/Part:BBa_K259007 main page]).
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4. OmpA-linker-Gli1 fusion protein controlled by arabinose regulated promoter (AraC/pBAD), [http://partsregistry.org/Part:BBa_K618111 (main page)]: This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Gli-1 DNA binding domain, BBa_K165007 ([http://partsregistry.org/Part:BBa_K165007 main page]), controlled by the arabinose-regulated promoter, Bba_K259007 ([http://partsregistry.org/Part:BBa_K259007 main page]).
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'''Data For Pre-existing Parts'''
 
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1. AIDA transmembrane domain, [[BBa_XXXXXX (Experience)]]
 
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2. K113009 (araC/ pBAD)
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'''Data For Pre-existing Parts'''
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1. arabinose regulated promoter, Bba_K259007 [http://partsregistry.org/Part:BBa_K259007:Experience (Experience)]
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'''We've Also Characterized the Following Parts'''
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===Parts===
===Parts===
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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<groupparts>iGEM011 Michigan</groupparts>
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<groupparts>iGEM010 Michigan</groupparts>
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Latest revision as of 22:53, 28 September 2011

Data Page

Schematic for Our System


Michigan 2011 pixcell scheme.png

Data For Our Favorite New Parts

1. OmpA-linker-Zif268 fusion protein controlled by LacI regulated promoter, [http://partsregistry.org/Part:BBa_K618212 (main page)]: This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Zif268-HIV DNA binding domain, BBa_K165006 ([http://partsregistry.org/Part:BBa_K165006 main page]), controlled by the LacI regulated promoter, BBa_J04500 ([http://partsregistry.org/Part:BBa_J04500 main page]).


2. OmpA-linker-Gli1 fusion protein controlled by LacI regulated promoter, [http://partsregistry.org/Part:BBa_K618211 (main page)]: This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Gli-1 DNA binding domain, BBa_K165007 ([http://partsregistry.org/Part:BBa_K165007 main page]), controlled by the LacI regulated promoter, BBa_J04500 ([http://partsregistry.org/Part:BBa_J04500 main page]).


3. OmpA-linker-Zif268 fusion protein controlled by arabinose regulated promoter (AraC/pBAD), [http://partsregistry.org/Part:BBa_K618112 (main page)]: This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Zif268-HIV DNA binding domain, BBa_K165006 ([http://partsregistry.org/Part:BBa_K165006 main page]), controlled by the arabinose-regulated promoter, Bba_K259007 ([http://partsregistry.org/Part:BBa_K259007 main page]).


4. OmpA-linker-Gli1 fusion protein controlled by arabinose regulated promoter (AraC/pBAD), [http://partsregistry.org/Part:BBa_K618111 (main page)]: This is a fusion protein assembled from OmpA-linker fusion protein, BBa_K103006 ([http://partsregistry.org/Part:BBa_K103006 main page]), and Gli-1 DNA binding domain, BBa_K165007 ([http://partsregistry.org/Part:BBa_K165007 main page]), controlled by the arabinose-regulated promoter, Bba_K259007 ([http://partsregistry.org/Part:BBa_K259007 main page]).



Data For Pre-existing Parts

1. arabinose regulated promoter, Bba_K259007 [http://partsregistry.org/Part:BBa_K259007:Experience (Experience)]



Parts

<groupparts>iGEM011 Michigan</groupparts>

Retrieved from "http://2011.igem.org/Team:Michigan/Parts"