Latest revision as of 22:31, 28 September 2011

Protocols

Basic protocols ::: These protocols are necessary for all lab operations.

Gels and electrophoresis ::: These protocols contain information on the production and usage of gels.

Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.

Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.

Biobrick specific ::: These protocols contain information specific to the usage of Biobricks.

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 =Protocols=
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 [[Media: electroporate.pdf | Electroporation of electrocompetent cells]]
-[[Media: competent.pdf | Production of standard competent cells]]
+[[Media: competent.pdf | Production of standard/QT competent cells]]
 [[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]]
+[[Media: heat_shock.pdf | Heat shock transformation of standard competent cells.]]
 '''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.
+[[Media: quick-ligase.pdf | Using quick ligase]]
+[[Media: T4-ligase.pdf | Using T4 ligase]]
+[[Media: digests.pdf | Using Restriction enzymes]]
+'''Biobrick specific''' ::: These protocols contain information specific to the usage of Biobricks.
+[[Media: biobrick_removal.pdf | Extraction of BioBricks from wells in the distribution]]
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