Team:Michigan/Protocols

From 2011.igem.org

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All protocols used should be aggregated here.
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=Protocols=
=Protocols=
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'''Fundamental protocols''':::These protocols are necessary for all lab operations. As many of the subjects are general, the protocols may contain few specific steps.
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'''Basic protocols''' ::: These protocols are necessary for all lab operations.  
[[Media: Autoclaving100.pdf | Autoclave usage]]
[[Media: Autoclaving100.pdf | Autoclave usage]]
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[[Media: OD600_100.pdf | OD600 optimization]]
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'''Basic lab protocols''':::Most laboratory operations will require the usage of these protocols.  
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'''Gels and electrophoresis''' ::: These protocols contain information on the production and usage of gels.
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[[Media: Electrophoresis102.pdf | Gel electrophoresis]]
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[[Media: Gel_viewer.pdf | Using the gel viewer]]
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'''Transformation''' ::: These protocols contain information on the production, usage, and transformation of competent cells.
[[Media: electrocompetent.pdf | Production of electrocompetent cells]]
[[Media: electrocompetent.pdf | Production of electrocompetent cells]]
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[[Media: electroporate.pdf | Electroporation of electrocompetent cells]]
[[Media: electroporate.pdf | Electroporation of electrocompetent cells]]
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[[Media: competent.pdf | Production of standard competent cells]]
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[[Media: competent.pdf | Production of standard/QT competent cells]]
[[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]]
[[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]]
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[[Media: heat_shock.pdf | Heat shock transformation of standard competent cells.]]
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'''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.
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[[Media: quick-ligase.pdf | Using quick ligase]]
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[[Media: T4-ligase.pdf | Using T4 ligase]]
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[[Media: digests.pdf | Using Restriction enzymes]]
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'''Biobrick specific''' ::: These protocols contain information specific to the usage of Biobricks.
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[[Media: biobrick_removal.pdf | Extraction of BioBricks from wells in the distribution]]
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{{:Team:Michigan/Template:Mich_Footer}}

Latest revision as of 22:31, 28 September 2011

Protocols

Basic protocols ::: These protocols are necessary for all lab operations.

Autoclave usage

OD600 optimization


Gels and electrophoresis ::: These protocols contain information on the production and usage of gels.

Gel electrophoresis

Using the gel viewer


Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.

Production of electrocompetent cells

Electroporation of electrocompetent cells

Production of standard/QT competent cells

Quick Transformation (QT) of standard competent cells

Heat shock transformation of standard competent cells.


Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.

Using quick ligase

Using T4 ligase

Using Restriction enzymes


Biobrick specific ::: These protocols contain information specific to the usage of Biobricks.

Extraction of BioBricks from wells in the distribution

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