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Revision as of 22:26, 28 September 2011

Some aditional stuff.

• Home made quick saliva DNA extraction protocol:

1. Rinse the mouth 5 times with clean water, the last rinse must be deposited in a plastic glass
2. Prepare a solution with soap and water in a beaker
3. Add the soap solution to the last rinse
4. Add frozen (0°C) etanol to the last rinse
5. Observe how a DNA thread forms, take it and place it in an eppendorf tube.

• Abbreviations and glossary:

• Biobrickoatl:It's the biobrick deity for the Mesoamerican cultures, although at that time they didn't knew what a biobrick meant. The great feathered biobrick serpent vigilates over all new and old biobricks.
• Competent cells: Cells that can incorporate, in an easier way, foreign DNA into their cell walls.
• CcaS-EnvZ: Is the green light receptor that induces the expresión of phycobilisome linker protein.
• CFP: Cyan Fluorescent Protein
• Crst: Lock for the PbadST (strong, for low concentrations) promoter
• CrWk: Lock for the Pbad WK (weak, for high concentration) promoter
• GFP: Green Fluorescent Protein
• ho1: Heme oxigenase 1
• iTaST: Inverse TaST, complementary sequence to the Key TaST, blocks TaST activity
• pBADST: Strong arabinose promoter for low concentration detection.
• pBADWk: Weak arabinose promoter for high concentration detection.
• Pcy4A: Phycocyanobilin ferredoxin oxidoreductase.
• RBS: Ribosome binding site
• TaST: Key for the CrST lock
• TaWk: Key for the CrWk lock
• Term: Terminator