Team:Wisconsin-Madison/ca

From 2011.igem.org

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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Project</a> >>  
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/main">Main</a> >>  
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>,  
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGem</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>,  
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ca">Contributions & Attributions</a>,  
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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Alkane Sensor
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Contributions and Attributions
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For this project, all cloning, testing, and web design has been done by the 2011 Wisconsin-Madison iGEM team.
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Parts originating from outside of the team and the registry include:
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- exaDE, which was codon optimized and synthesized by order from GeneArt
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- tagRFP, which was provided by our advisors' lab and was originally created by Evrogen
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In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of <i>E. coli.</i> To develop an alkane biosensor, <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> from <i>Pseudomonas putida</i> and <i>Alcanivorax borkumensis</i> were isolated and constructed into a pair of <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmids">plasmids</a> which code for proteins that bind to alkanes and then induce a promoter upstream of a red fluorescent protein. After gathering data at several different concentrations of alkane, a linear regression was produced, allowing for the quantification of an unknown ethanol concentration in a media based upon the level of fluorescence. 
 
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Learn more about: <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a>, <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmids">plasmids</a>.
 

Latest revision as of 22:06, 28 September 2011









Main >> What is iGem, Contributions & Attributions,

Contributions and Attributions

For this project, all cloning, testing, and web design has been done by the 2011 Wisconsin-Madison iGEM team.

Parts originating from outside of the team and the registry include:

- exaDE, which was codon optimized and synthesized by order from GeneArt

- tagRFP, which was provided by our advisors' lab and was originally created by Evrogen



Retrieved from "http://2011.igem.org/Team:Wisconsin-Madison/ca"