Team:UTP-Panama/Week 15

From 2011.igem.org

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==September 12==
==September 12==
===WET LAB===
===WET LAB===
-
(Objetives or Title):
+
We grew the BBa-K381001 in liquid LB + chloramphenicol to then continue with DIRTY MINIPREP in order to extract its plasmids.
-
--ESCRIBIR Y MEJORAR LAB--
+
#Took out of the fridge 2 petri dishes with transformed bacteria with BBa-K381001
 +
#It was used:
 +
##9.7µL of chloramphenicol
 +
##and 10mL of liquid LB
 +
#Prepared (+) and (-) controls for each petri dish in falcon tubes
 +
#Each falcon tube of (+) control contains:
 +
##9.7µL of chloramphenicol/10mL of Liquid LB
 +
##5mL of liquid LB
 +
##Aliquot of the cultivation
 +
#The negative controls had almost the same content, excluding chloramphenicol
 +
#The 4 falcon tubes were collocated in the incubator until next day
 +
 
 +
 
 +
==September 13==
-
==September 13==}
 
===Scientific Comunication Activities===
===Scientific Comunication Activities===
Presentation (by the students of iGEM UTP-Panama) in the Civil and Electrical Faculties  about:<br>
Presentation (by the students of iGEM UTP-Panama) in the Civil and Electrical Faculties  about:<br>

Revision as of 21:43, 28 September 2011


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Week 15: September 12 to 17

September 12

WET LAB

We grew the BBa-K381001 in liquid LB + chloramphenicol to then continue with DIRTY MINIPREP in order to extract its plasmids.

  1. Took out of the fridge 2 petri dishes with transformed bacteria with BBa-K381001
  2. It was used:
    1. 9.7µL of chloramphenicol
    2. and 10mL of liquid LB
  3. Prepared (+) and (-) controls for each petri dish in falcon tubes
  4. Each falcon tube of (+) control contains:
    1. 9.7µL of chloramphenicol/10mL of Liquid LB
    2. 5mL of liquid LB
    3. Aliquot of the cultivation
  5. The negative controls had almost the same content, excluding chloramphenicol
  6. The 4 falcon tubes were collocated in the incubator until next day


September 13

Scientific Comunication Activities

Presentation (by the students of iGEM UTP-Panama) in the Civil and Electrical Faculties about:
What's SynBio?
Applications of SynBio
What's iGEM?
What are we doing? (our project)
Future projects

WET LAB

(Objetives or Title): --ESCRIBIR Y MEJORAR LAB--

September 14

WET LAB

(Objetives or Title): --ESCRIBIR Y MEJORAR LAB--

September 15

WET LAB

(Objetives or Title): --ESCRIBIR Y MEJORAR LAB--

September 16

WET LAB

(Objetives or Title): --ESCRIBIR Y MEJORAR LAB--

September 17

GENERAL SESSION

Morning: Talikng about WET LAB final activities. All the Team (under Human Practice Direction):

Schematization of algorithms and flow chart for a Human Practice Project.
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