From 2011.igem.org
(Difference between revisions)
|
|
| (4 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | -Spin 1.5 mL of overnight culture in microfuge </br>
| + | <gallery widths=910px heights=800px> |
| - | -Aspirate all but 100uL of the supernatant and resuspend pellet by vortexing</br>
| + | File:June7GDS.jpg |
| - | -Add 300uL of TENS and mix by inversion</br>
| + | </gallery> |
| - | -Add 150uL of sodium acetate and vortex</br>
| + | |
| - | -Centrifuge for 2.5 minutes at 10K</br>
| + | |
| - | -Transfer supernatant to clean tube and add 1mL of room temp. ETOH</br>
| + | |
| - | -Mix and pellet DNA by centrifuge for 2-5 min. at 10K</br>
| + | |
| - | -Wash pellet with 70% ethanol and allow pellet to dry</br>
| + | |
| - | -Resuspend pellet in 30uL of TE w/ RNA seA</br>
| + | |
| - | -Digest 5-10uL as usual</br>
| + | |
| | | | |
| - | | + | <div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:gelpicnewerGDS.jpg|425px]] |
| - | :To make TENS, add: | + | </div> |
| - | ::-4.5mL of TE | + | |
| - | :::-250uL 10%SDS
| + | |
| - | :::-250uL 2N NaOH
| + | |
Latest revision as of 20:43, 28 September 2011
"
