Team:Lethbridge/Attributions
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=Collaboration - Calgary iGEM= | =Collaboration - Calgary iGEM= | ||
- | We were asked by the Calgary iGEM team | + | We were asked by the Calgary iGEM team to test the viability of their chassis to survive in tailings pond water. If <i>E. coli</i> DH5α cells containing one of our constructs can grow normally in medium prepared with tailings water, we can be confident that the chassis can be applied to tailings pond water in general. |
===Materials and Methods=== | ===Materials and Methods=== | ||
50 mL of LB medium was made with tailings water and filtered through MILLEX filter Unit GS MF-Millipore MCE Membrane 0.22 µm in order to sterilize it and appropriate antibiotics were added. The tailings LB was inoculated with <i>E. coli</i> DH5α cells containing BBa_K331009 to an OD<sub>600</sub> of 0.1. The flask was incubated at 37˚C with shaking for 7 hours, during which photometric readings were taken every 30. Photometric readings were taken using the Pharmacia Biotech Ultrospec 3000, as the OD<sub>600</sub> readings reached 1.0 the solutions was diluted with LB media to keep the OD<sub>600</sub> reading between 0.1 and 1.0. The same protocol was used to observe cell growth of <i>E. coli</i> DH5α cells containing BBa_K331009 in 50 mL of LB medium made with MilliQ H<sub>2</sub>O. | 50 mL of LB medium was made with tailings water and filtered through MILLEX filter Unit GS MF-Millipore MCE Membrane 0.22 µm in order to sterilize it and appropriate antibiotics were added. The tailings LB was inoculated with <i>E. coli</i> DH5α cells containing BBa_K331009 to an OD<sub>600</sub> of 0.1. The flask was incubated at 37˚C with shaking for 7 hours, during which photometric readings were taken every 30. Photometric readings were taken using the Pharmacia Biotech Ultrospec 3000, as the OD<sub>600</sub> readings reached 1.0 the solutions was diluted with LB media to keep the OD<sub>600</sub> reading between 0.1 and 1.0. The same protocol was used to observe cell growth of <i>E. coli</i> DH5α cells containing BBa_K331009 in 50 mL of LB medium made with MilliQ H<sub>2</sub>O. |
Revision as of 19:39, 28 September 2011
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