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Team:Caltech/Recipes
From 2011.igem.org
(Difference between revisions)
| Line 22: | Line 22: | ||
* 10 mL Wolfe's Vitamin Solution | * 10 mL Wolfe's Vitamin Solution | ||
* 1 mL SL-10 Trace Element Solution. | * 1 mL SL-10 Trace Element Solution. | ||
| - | * variable amounts of EDC's}} | + | * variable amounts of EDC's |
| + | <br/> | ||
| + | |||
| + | <p>'''SOC Media'''</p> | ||
| + | *for 250 ml (adapted from [http://openwetware.org/wiki/SOC OpenWetWare]) | ||
| + | *1.25 g yeast extract | ||
| + | *5 g tryptone | ||
| + | *0.146 g NaCl | ||
| + | *0.0465 g KCl | ||
| + | *0.616 g MgSO4*7H2O | ||
| + | *0.508 g MgCl2*6H2O | ||
| + | *adjust pH to 7.5 using 1M NaOH | ||
| + | *autoclave | ||
| + | *after cooling below 50 degrees Celsius, add 20mL filter-sterilized 20% glucose solution.}} | ||
Revision as of 19:49, 24 June 2011
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Project |
Agar/LB plate (Autoclaved):
50% glycerol Stock:
Enrichment Minimal Media
SOC Media
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