Team:Arizona State/Templates/E coli contingency/table 4

From 2011.igem.org

(Difference between revisions)
(Created page with "{| class="wikitable" style="text-align:center; width:80%;" |+ Transformation Efficiency Results ! style="background: #ffdead;" | E. Coli Strain ! style="background: #ffdea...")
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! style="background: #ffdead;" | DNA Quantity Used (ng)
! style="background: #ffdead;" | DNA Quantity Used (ng)
! style="background: #ffdead;" | # of Colonies
! style="background: #ffdead;" | # of Colonies
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! style="background: #ffdead;" | Weighted Colony Count*
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! style="background: #ffdead;" | Weighted Colony Count**
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| MG1655 || GFP Construct in pRSF || Leader in pIDT || 140 || 21 || 138.742
| MG1655 || GFP Construct in pRSF || Leader in pIDT || 140 || 21 || 138.742
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| BL21 || Leader+Seq1 in pIDT || GFP Construct in pRSF || 143.4 || 9 || 35.186
| BL21 || Leader+Seq1 in pIDT || GFP Construct in pRSF || 143.4 || 9 || 35.186
|}
|}
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<small>''*Weighted Colony Count = (# of Colonies)/(mean OD600 of competency prep)''</small>
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<small>''**Weighted Colony Count = (# of Colonies)/(mean OD600 of competency prep)''</small>

Revision as of 13:00, 28 September 2011

Transformation Efficiency Results
E. Coli Strain Initial Plasmid Introduced Plasmid DNA Quantity Used (ng) # of Colonies Weighted Colony Count**
MG1655 GFP Construct in pRSF Leader in pIDT 140 21 138.742
MG1655 GFP Construct in pRSF Leader+Seq1 in pIDT 147 31 204.810
MG1655 Leader in pIDT GFP Construct in pRSF 143.4 23 117.635
MG1655 Leader+Seq1 in pIDT GFP Construct in pRSF 143.4 1000 3909.508
BL21 GFP Construct in pRSF Leader in pIDT 140 0 0.000
BL21 GFP Construct in pRSF Leader+Seq1 in pIDT 147 2 11.574
BL21 Leader in pIDT GFP Construct in pRSF 143.4 14 60.512
BL21 Leader+Seq1 in pIDT GFP Construct in pRSF 143.4 9 35.186

**Weighted Colony Count = (# of Colonies)/(mean OD600 of competency prep)

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